概述

  • 产品名称Anti-Hsc70抗体[1B5] (Biotin)
    参阅全部 Hsc70 一抗
  • 描述
    大鼠单克隆抗体[1B5] to Hsc70 (Biotin)
  • 偶联物Biotin
  • 特异性ab69558 is highly specific for Hsc70 under both native and denaturing conditions and will not react with the inducible Hsp70 (hsp72).
  • 经测试应用适用于: IHC-Fr, ELISA, WB, IP, ICC, Electron Microscopy, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Monkey
  • 免疫原

    Full length Hsc70 protein purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells.

  • 阳性对照
    • Human HeLa cell lysate. Recombinant Bovine Hsc70 Protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 0.15M PBS, 0.1M Sodium chloride, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody was purified using Protein G chromatography prior to biotinylation.
  • 克隆单克隆
  • 克隆编号1B5
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ELISA 1/100 - 1/750.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.
IP Use a concentration of 5 µg/ml.
ICC Use a concentration of 5 µg/ml.
Electron Microscopy 1/1000.
Flow Cyt Use at an assay dependent concentration. ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the heat shock protein 70 family.
  • 结构域The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • 细胞定位Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2410008N15Rik antibody
    • Constitutive heat shock protein 70 antibody
    • Epididymis luminal protein 33 antibody
    • Epididymis secretory sperm binding protein Li 72p antibody
    • Heat shock 70 kDa protein 8 antibody
    • Heat shock 70kD protein 10 antibody
    • Heat shock 70kD protein 8 antibody
    • Heat shock 70kDa protein 8 antibody
    • Heat shock cognate 71 kDa protein antibody
    • Heat shock cognate protein 54 antibody
    • Heat shock cognate protein 71 kDa antibody
    • Heat shock protein 8 antibody
    • Heat shock protein A8 antibody
    • Heat-shock70-KD protein 10, formerly antibody
    • HEL 33 antibody
    • HEL S 72p antibody
    • HSC54 antibody
    • HSC71 antibody
    • Hsc73 antibody
    • HSP71 antibody
    • HSP73 antibody
    • HSP7C_HUMAN antibody
    • HSPA10 antibody
    • HSPA8 antibody
    • LAP1 antibody
    • Lipopolysaccharide associated protein 1 antibody
    • LPS associated protein 1 antibody
    • LPS associated protein antibody
    • MGC102007 antibody
    • MGC106514 antibody
    • MGC114311 antibody
    • MGC118485 antibody
    • MGC131511 antibody
    • MGC29929 antibody
    • N-myristoyltransferase inhibitor protein 71 antibody
    • NIP71 antibody
    see all

Anti-Hsc70 antibody [1B5] (Biotin) (ab69558)参考文献

This product has been referenced in:
  • Traister A  et al. Mutation in integrin-linked kinase (ILK(R211A)) and heat-shock protein 70 comprise a broadly cardioprotective complex. PLoS One 8:e77331 (2013). WB ; Human . Read more (PubMed: 24260102) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"