概述

  • 产品名称
    Anti-Hsc70抗体[1B5]
    参阅全部 Hsc70 一抗
  • 描述
    大鼠单克隆抗体[1B5] to Hsc70
  • 经测试应用
    适用于: ICC/IF, IHC-P, Flow Cyt, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Monkey
  • 免疫原

    Full length native protein (purified) corresponding to Hamster Hsc70.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    Constituents: PBS, 50% Glycerol, 0.0017% PMSF
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    1B5
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab19136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 73 kDa.
IP Use a concentration of 5 µg/ml.

靶标

  • 功能
    Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Belongs to the heat shock protein 70 family.
  • 结构域
    The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • 细胞定位
    Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2410008N15Rik antibody
    • Constitutive heat shock protein 70 antibody
    • Epididymis luminal protein 33 antibody
    • Epididymis secretory sperm binding protein Li 72p antibody
    • Heat shock 70 kDa protein 8 antibody
    • Heat shock 70kD protein 10 antibody
    • Heat shock 70kD protein 8 antibody
    • Heat shock 70kDa protein 8 antibody
    • Heat shock cognate 71 kDa protein antibody
    • Heat shock cognate protein 54 antibody
    • Heat shock cognate protein 71 kDa antibody
    • Heat shock protein 8 antibody
    • Heat shock protein A8 antibody
    • Heat shock protein family A (Hsp70) member 8 antibody
    • Heat-shock70-KD protein 10, formerly antibody
    • HEL 33 antibody
    • HEL S 72p antibody
    • HSC54 antibody
    • HSC71 antibody
    • Hsc73 antibody
    • HSP71 antibody
    • HSP73 antibody
    • HSP7C_HUMAN antibody
    • HSPA10 antibody
    • HSPA8 antibody
    • LAP1 antibody
    • Lipopolysaccharide associated protein 1 antibody
    • LPS associated protein 1 antibody
    • LPS associated protein antibody
    • MGC102007 antibody
    • MGC106514 antibody
    • MGC114311 antibody
    • MGC118485 antibody
    • MGC131511 antibody
    • MGC29929 antibody
    • N-myristoyltransferase inhibitor protein 71 antibody
    • NIP71 antibody
    see all

Anti-Hsc70 antibody [1B5] 图像

  • All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 0.1 µg/ml

    Lane 1 : G361 human melanoma cells - whole cell lysate (50000 cells/lane)
    Lane 2 : T47D human breast cancer cells - whole cell lysate (50000 cells/lane)

    Secondary
    HRP conjugated rabbit anti-rat IgG
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 73 kDa
    Observed band size : 73 kDa


    Exposure time : 15 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab19136 staining Hsc70 in Human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/150) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rat polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 1/20000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HEC59 whole cell lysate
    Lane 3 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat anti-ray HRP conjugate at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 73 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 seconds

    This image is courtesy of an anonymous abreview.

    Blocking: 5% milk for 1 hour at 22°C.

    See Abreview

  • All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 0.1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Hsc70 (Hsp73) recombinant protein
    Lane 3 : Hsp70 (Hsp72) protein
    Lane 4 : Cell lysates prepared from 3T3 cells
    Lane 5 : Cell lystes prepared from human PC-12 cells
    Lane 6 : Cell lysates prepared from CHO cells
    Lane 7 : Cell lysates prepared from GPC-16 cells
    Lane 8 : Cell lysates prepared from human Hela cells

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 73 kDa
  • ab19136 at 1/150 dilution staining human HeLa cells by ICC/IF. The cells were heat shocked (2 hours at 42.5°C) and paraformaldehyde fixed prior to incubation with the antibody for 1 hour at 37°C. An Alexa-Fluor ® 568 conjugated goat anti-rat antibody was used as the secondary. The cells were also stained with DAPI to visualize the nuclei.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab19136  (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19136, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Hsc70 antibody [1B5] (ab19136)参考文献

This product has been referenced in:
  • Miles AL  et al. The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1a prolyl hydroxylation by regulating cellular iron levels. Elife 6:N/A (2017). WB ; Human . Read more (PubMed: 28296633) »
  • Wang B  et al. Essential control of mitochondrial morphology and function by chaperone-mediated autophagy through degradation of PARK7. Autophagy 12:1215-28 (2016). Read more (PubMed: 27171370) »

See all 19 Publications for this product

Product Wall


According to the protein database entry http://www.uniprot.org/uniprot/P11142
the protein is located as follows:

Cytoplasm. Melanosome. Nucleus › nucleolus. Note: Localized in cytoplasmic mRNP granules containing untranslated m...

Read More

Thank you very much for your interest in our products.

We do not offer free or trial sizes of our products simply because we carry over 100,000 items and having multiple sizes would be logistically impossible with our automated freezers. Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela, HEC59, HCT116)
Loading amount
30 µg
Specification
Hela, HEC59, HCT116
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast)
Specification
Breast
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma (G361), breast cancer (T47D) cell lines)
Loading amount
50000 cells
Specification
melanoma (G361), breast cancer (T47D) cell lines
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Oct 09 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Specification
Hela
Fixative
Paraformaldehyde
Blocking step
Other as blocking agent for 30 minute(s) · Concentration: n/a
Username

Abcam user community

Verified customer

提交于 Sep 29 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cultured hippocampal neurons)
Specification
cultured hippocampal neurons
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1%
Username

Dr. Vladimir Sytnyk

Verified customer

提交于 Aug 07 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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