Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HS6ST1.
(Peptide available as ab69681.)
- This antibody gave a positive signal in the following tissue lysates:
Human Fetal Liver; Human Brain
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).
6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.
Expressed in fetal brain.
Belongs to the sulfotransferase 6 family.
Information by UniProt
- 6OST 1 antibody
- 6OST1 antibody
- DKFZp547H098 antibody
Western blot - HS6ST1 antibody (ab69682)
All lanes :
Anti-HS6ST1 antibody (ab69682) at 1 µg/mlLane 1 :
Liver (Human) Tissue Lysate - fetal normal tissue (ab29890
)Lane 2 :
Human brain tissue lysate - total protein (ab29466
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.Predicted band size:
48 kDaObserved band size:
47 kDa (why is the actual band size different from the predicted?
)Additional bands at:
43 kDa, 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.Exposure time:
has not yet been referenced specifically in any publications.
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