概述

  • 产品名称Anti-HRP抗体
    参阅全部 HRP 一抗
  • 描述
    大鼠多克隆抗体to HRP
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues may occur but have not been specifically determined.
  • 经测试应用适用于: Dot blot, WB, ELISA, IPmore details
  • 种属反应性
    Reacts with Horseradish.
  • 免疫原

    Full length Horseradish Peroxidase.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Constituents: 0.87% Sodium chloride, 0.164% Sodium phosphate
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by a delipidation and defibrination.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot
WB
ELISA
IP
  • 应用说明Dot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/6000. This product has been assayed against 1.0µg of Peroxidase [Horseradish] in a standard sandwich ELISA using Alklaine Phosphatase conjugated affinity purified anti-Rat IgG [H&L] (Goat) and pNPP as a substrate for 30 minutes at RT.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.

    Suitable for most immunological methods requiring high titer and specificity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
    • 细胞定位Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
    • 别名
      • Horseradish Peroxidase antibody
      • HPRC1 antibody
      • HRP antibody
      • Peroxidase antibody
      • Peroxidase C1A antibody
      • PRXC1A antibody
      see all

    Anti-HRP antibody (ab34827)参考文献

    ab34827 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"