Anti-HRP抗体[2H11] (ab10183)
Key features and details
- Mouse monoclonal [2H11] to HRP
- Suitable for: ELISA, IHC-Fr, IHC-P
- Reacts with: Horseradish
- Isotype: IgG2b
概述
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产品名称
Anti-HRP抗体[2H11]
参阅全部 HRP 一抗 -
描述
小鼠单克隆抗体[2H11] to HRP -
宿主
Mouse -
特异性
Horseradish peroxidase isoforms with pI from 3.5 to 9.5. ab10183 does not influence enzymatic activity upon immune complex formation. -
经测试应用
适用于: ELISA, IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Horseradish -
免疫原
Full length native protein (purified) corresponding to HRP.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis. -
克隆
单克隆 -
克隆编号
2H11 -
骨髓瘤
Sp2/0 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10183于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
说明 |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
靶标
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相关性
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. -
细胞定位
Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles. -
数据库链接
- SwissProt: P00433 Horseradish
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别名
- Horseradish Peroxidase antibody
- HPRC1 antibody
- HRP antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab10183 被引用在 10 文献中.
- Du J Synergistic effect of Bruton's tyrosine kinase and TNF-α in the regulation of rheumatoid arthritis and underlying mechanisms. Exp Ther Med 23:141 (2022). PubMed: 35069822
- Zhang B et al. Up-regulation of miR-204 inhibits proliferation, invasion and apoptosis of gallbladder cancer cells by targeting Notch2. Aging (Albany NY) 13:2941-2958 (2021). PubMed: 33460397
- Wang Z et al. Long noncoding RNA PTENP1 affects the recovery of spinal cord injury by regulating the expression of miR-19b and miR-21. J Cell Physiol 235:3634-3645 (2020). PubMed: 31583718
- Sharafeldin M et al. Influence of antibody immobilization strategy on carbon electrode immunoarrays. Analyst 144:5108-5116 (2019). PubMed: 31373337
- Liu B et al. Effect of microRNA-27b on cisplatin chemotherapy sensitivity of oral squamous cell carcinoma via FZD7 signaling pathway. Oncol Lett 18:667-673 (2019). PubMed: 31289540
- Lin TY et al. Mapping chromatic pathways in the Drosophila visual system. J Comp Neurol 524:213-27 (2016). PubMed: 26179639
- Miyazaki T et al. A gustatory second-order neuron that connects sucrose-sensitive primary neurons and a distinct region of the gnathal ganglion in the Drosophila brain. J Neurogenet 29:144-55 (2015). PubMed: 26004543
- Shazeeb MS et al. Targeted signal-amplifying enzymes enhance MRI of EGFR expression in an orthotopic model of human glioma. Cancer Res 71:2230-9 (2011). IHC-Fr . PubMed: 21245103
- Terashima H et al. A sensitive short-term evaluation of antifibrotic effects using newly established type I collagen reporter transgenic rats. Am J Physiol Renal Physiol 299:F792-801 (2010). IHC-Fr . PubMed: 20660018
- He DF et al. Morpho-Physiologic Characteristics of Dorsal Subicular Network in Mice after Pilocarpine-Induced Status Epilepticus. Brain Pathol : (2009). IHC . PubMed: 19298597