概述

  • 产品名称
    Anti-HRP抗体[2H11]
    参阅全部 HRP 一抗
  • 描述
    小鼠单克隆抗体[2H11] to HRP
  • 特异性
    Horseradish peroxidase isoforms with pI from 3.5 to 9.5. ab10183 does not influence enzymatic activity upon immune complex formation.
  • 经测试应用
    适用于: ELISA, IHC-P, IHC-Frmore details
  • 免疫原

    Purified peroxidase from horseradish.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • 克隆
    单克隆
  • 克隆编号
    2H11
  • 骨髓瘤
    Sp2/0
  • 同种型
    IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性
    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
  • 细胞定位
    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
  • 别名
    • Horseradish Peroxidase antibody
    • HPRC1 antibody
    • HRP antibody
    • Peroxidase antibody
    • Peroxidase C1A antibody
    • PRXC1A antibody
    see all

文献

This product has been referenced in:
  • Shazeeb MS  et al. Targeted signal-amplifying enzymes enhance MRI of EGFR expression in an orthotopic model of human glioma. Cancer Res 71:2230-9 (2011). IHC-Fr . Read more (PubMed: 21245103) »
  • Terashima H  et al. A sensitive short-term evaluation of antifibrotic effects using newly established type I collagen reporter transgenic rats. Am J Physiol Renal Physiol 299:F792-801 (2010). IHC-Fr . Read more (PubMed: 20660018) »

See all 3 Publications for this product

客户评价及客户问答

Thank you for your enquiry. Unfortunately, the HRP epitope that this antibody recognizes has not been determined. However, it is likely to recognizes the protein part of the molecule because it reacts with various peroxidase strains differing in com...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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