RabMAb

Anti-HRH3抗体[EPR5631] (ab124732)

概述

  • 产品名称Anti-HRH3抗体[EPR5631]
    参阅全部 HRH3 一抗
  • 描述
    兔单克隆抗体[EPR5631] to HRH3
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HRH3 (N terminal). The exact sequence is proprietary. (In the extracellular domain).
    Database link: Q9Y5N1

  • 阳性对照
    • Lysates of: Jurkat, SH-SY5Y, C6, A549, Neuro-2a Jurkat cells
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 解离常数(KD KD = 2.62 x 10 -10 M Learn more about KD
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR5631
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization.
    • 组织特异性Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra.
    • 序列相似性Belongs to the G-protein coupled receptor 1 family.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • G protein coupled receptor 97 antibody
      • G-protein coupled receptor 97 antibody
      • GPCR 97 antibody
      • GPCR97 antibody
      • H3R antibody
      • HH 3R antibody
      • HH3 R antibody
      • HH3R antibody
      • Histamine H3 receptor antibody
      • Histamine receptor H3 antibody
      • HRH 3 antibody
      • HRH3 antibody
      • HRH3_HUMAN antibody
      • OTTHUMP00000031459 antibody
      see all

    Anti-HRH3 antibody [EPR5631] 图像

    • All lanes : Anti-HRH3 antibody [EPR5631] (ab124732)

      Lane 1 : Jurkat cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 49 kDa
    • Flow cytometric analysis of Jurkat cells using ab124732 (red)at 1/100 dilution or a rabbit IgG (negative) (green)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-HRH3 antibody [EPR5631] (ab124732)参考文献

    ab124732 has not yet been referenced specifically in any publications.

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