HRG overexpression 293T裂解液(全细胞) (ab94198)

概述

  • 产品名称HRG overexpression 293T裂解液(全细胞)
  • 描述
    HRG overexpression裂解液
  • 常规说明ab94198 is a 293T cell transfected lysate in which Human HRG has been transiently over-expressed using a pCMV-HRG plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景HRG (histidine-rich glycoprotein) contains two cystatin-like domains and is located in plasma and platelets. The physiological function is not yet known. It binds heme, dyes and divalent metal ions. It can inhibit rosette formation and interacts with heparin, thrombospondin, and the lysine-binding site of plasminogen. A potential prothrombotic effect of HRG is indicated by the inhibition of fibrinolysis and the reduction of inhibition of coagulation. Mutations in HRG lead to thrombophilia due to abnormal histidine-rich glycoprotein levels.

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应用

Our Abpromise guarantee covers the use of ab94198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

HRG overexpression 293T lysate (whole cell) 图像

  • ab94198 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-HRG antibody (ab67807) at 1/500 dilution

    Lane 1 : HRG overexpression 293T lysate (whole cell) (ab94198)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

HRG overexpression 293T lysate (whole cell) (ab94198)参考文献

ab94198 has not yet been referenced specifically in any publications.

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