Anti-HRAS + KRAS抗体(ab53160)

概述

  • 产品名称Anti-HRAS + KRAS抗体
    参阅全部 HRAS + KRAS 一抗
  • 描述
    兔多克隆抗体to HRAS + KRAS
  • 特异性Detects endogenous levels of total KRAS/HRAS protein
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human HRAS + KRAS.

  • 阳性对照
    • HeLa cell extract

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab53160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
ELISA 1/20000.

靶标

  • 相关性KRAS: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Enzyme regulation: Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Interaction with SOS1 promotes exchange of bound GDP by GTP. HRAS: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Enzyme regulation: Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
  • 数据库链接
  • 别名
    • c-H-ras antibody
    • c-K-ras antibody
    • c-Ki-ras antibody
    • GTPase HRas antibody
    • GTPase KRas antibody
    • H-Ras-1 antibody
    • Ha-Ras antibody
    • HRAS antibody
    • HRAS1 antibody
    • K-Ras 2 antibody
    • Ki-Ras antibody
    • KRAS antibody
    • KRAS2 antibody
    • p21ras antibody
    • RASK2 antibody
    • Transforming protein p21 antibody
    see all

Anti-HRAS + KRAS antibody 图像

  • All lanes : Anti-HRAS + KRAS antibody (ab53160) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with the immunising peptide

    Lysates/proteins at 5 µg per lane.

    Secondary
    Lane 1 : Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)
    Lane 2 : Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)


    Predicted band size : 22 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

Anti-HRAS + KRAS antibody (ab53160)参考文献

ab53160 has not yet been referenced specifically in any publications.

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