概述

  • 产品名称Anti-HPV18 E7抗体[8E2]
    参阅全部 HPV18 E7 一抗
  • 描述
    小鼠单克隆抗体[8E2] to HPV18 E7
  • 经测试应用适用于: WB, IP, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human Papillomavirus (HPV)
  • 免疫原

    Recombinant full length HPV18 E7 protein

  • 表位Mapped to amino acids 36-70
  • 阳性对照
    • WB: U20S, Hela and SiHa cell lysates ICC/IF: U2OS cells transfected with plasmid expressing HPV18 E7 protein; Hela cells.
  • 常规说明Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

性能

应用

Our Abpromise guarantee covers the use of ab100953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 12 kDa.
IP 1/500.
ELISA 1/128000.
ICC/IF Use at an assay dependent dilution.

靶标

  • 相关性Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
  • 数据库链接
    • 别名
      • E7 protein antibody
      • Human Papilloma Virus Type 18 E7 protein antibody

    Anti-HPV18 E7 antibody [8E2] 图像

    • All lanes : Anti-HPV18 E7 antibody [8E2] (ab100953) at 1/1000 dilution

      Lane 1 : lysate of U20S cells with plasmid expressing E7 protein
      Lane 2 : plain U20S lysate
      Lane 3 : Hela lysate
      Lane 4 : SiHa lysate
      Lane 5 : Marker


      Predicted band size : 12 kDa
    • IP analysis of HPV18 E7 protein using ab100953 at 1/500:
      Lane 1: detection of E7 protein in lysate of U20S cells
      Lane 2: the lower band is precipitated E7, upper bands are light and heavy chain of antibody
      Lane 3: precipitation without ab100953
      Lane 4: IP exp. with plain U2OS cells
      Lane 5: IP exp. with plain Hela cells, where the lower band is non specific binding
      Lane 6: marker.
    • Immunofluorescent (IF) analysis of HPV18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E7 detected by ab100953. IF analysis of E7 protein shows similar result also in HeLa cells.

    Anti-HPV18 E7 antibody [8E2] (ab100953)参考文献

    This product has been referenced in:
    • Ohba K  et al. In Vivo and In Vitro Studies Suggest a Possible Involvement of HPV Infection in the Early Stage of Breast Carcinogenesis via APOBEC3B Induction. PLoS One 9:e97787 (2014). ICC/IF ; Human . Read more (PubMed: 24858917) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - other (human foreskin keratinocytes)
    Loading amount 10 µg
    Specification human foreskin keratinocytes
    Gel Running Conditions Reduced Denaturing (15% Gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    提交于 Dec 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"