Anti-HPV16 E6 + HPV18 E6抗体[C1P5] (ab70)

Abreviews (2)Q&A (20)Specific References (15)

概述

  • 产品名称
    Anti-HPV16 E6 + HPV18 E6抗体[C1P5]
    参阅全部 HPV16 E6 + HPV18 E6 一抗
  • 描述
    小鼠单克隆抗体[C1P5] to HPV16 E6 + HPV18 E6
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 免疫原

    Gel-purified HPV-18 E6-beta galactosidase fusion protein.

  • 阳性对照
    • Human cervical cancer cells.
  • 常规说明

    Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.

    The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.

性能

应用

Our Abpromise guarantee covers the use of ab70 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 16.5 kDa.

16.5 kDa band is HPV-18, 17 kDa band is HPV-16.

The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性
    Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1,6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation.
  • 别名
    • E6 antibody
    • Human Papilloma Virus antibody
    • Human papillomavirus type 16 E6 antibody
    • Human papillomavirus type 18 E6 antibody
    • Protein E6 antibody
    see all

图片

  • Western blot - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)
    Western blot - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)Image from Zhang Y et al, J Biol Chem. 2005 Sep 30;280(39):33165-77. Epub 2005 Jun 27, Fig 9. DOI 10.1074/jbc.M505124200
    Subconfluent proliferating MCF-7 or T47D cells were transiently transfected overnight with empty pcDNA3 vector, with FLAG wild-type E6 or zinc finger defective mutant E6 (E6-(C66,136G)). The cells were washed, post-incubated for 24 hours to allow gene expression, and harvested for Western blotting to detect the E6 oncoprotein using ab70.

文献

This product has been referenced in:
  • Wang X  et al. Human papillomavirus type 16 E6 oncoprotein promotes proliferation and invasion of non-small cell lung cancer cells through Toll-like receptor 3 signaling pathway. J Med Virol N/A:N/A (2017). WB ; Human . Read more (PubMed: 28480962) »
  • Mitkevich VA  et al. Binase treatment increases interferon sensitivity and apoptosis in SiHa cervical carcinoma cells by downregulating E6 and E7 human papilloma virus oncoproteins. Oncotarget 8:72666-72675 (2017). Read more (PubMed: 29069817) »

See all 15 Publications for this product

发表研究结果有使用 ab70？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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22 reviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HPV16 E6 + HPV18 E6 antibody [C1P5]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Uterine cervix, Squamous cell carcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0
Permeabilization
No
Specification
Uterine cervix, Squamous cell carcinoma
Fixative
Formaldehyde
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Username

Mr. Allan Wang

Verified customer

提交于 Apr 17 2018

Does this antibody contain an Fc region?

Thank you for your inquiry

I heard back from the lab that, unfortunately, they do not have any information relating to the structure of the antibody. But this is a standard antibody so I would expect that is does have an Fc region.

...

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ab70: will test in ELISA
send DS link for ab98693

Thank you for your phone call.

The link to the datasheet of the secondary antibody is :

http://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-hrp-pre-adsorbed-ab98693.html


Here is the discount...

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Hi,

I would like to know the differences between the next two antibodies:

Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70) and

Anti-HPV18 E6 antibody [289-13965] (ab30716)

In both, the description suggest that...

Read More

Thank you for contacting us and your interest in our products.

The antibodies ab70 and ab30716 are in fact the same antibody, produced from different sources.I can understand that this is currently not reflected very well on our website but w...

Read More

Immunocytochemistry abreview for Anti-HPV16 E6 + HPV18 E6 antibody [C1P5]

 Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Cow Cultured Cells (Epithelial cells of cows with BPV and cells free-B)
Specification
Epithelial cells of cows with BPV and cells free-B
Fixative
Paraformaldehyde
Permeabilization
Yes - Tritonx100 (0,1%),
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Username

Abcam user community

Verified customer

提交于 Aug 31 2012

Our costumer told me that she had already submitted an ABReview for ab70 (ICC application), but today I was visiting ABCAM website and couldn't find it.

So, I would like to know from you:

1- When an ABReview was submitted does it go s...

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Thank you for your reply.


I have located the Abreview in our system for ab70. It seems that the customer has begun this review, but must provide the product lot number (found on the vial) in order to officially submit the review. Therefor...

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I had talked to our costumer and she has already submitted an ABReview for ab70 with her ICC/ IF results.

She is really happy with the discount for getting a new antibody for free.



She said that will talk to her Lab ma...

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Thank you for the update and I am really happy that the customer was satisfied with the outcome.

If you have any other situations like this or if there is anything else we can help with, then please let me know.

Was this antibody tested in ELISA to verify specificity? If so, what antigen was used?
What other QC tests were performed?

Thank you very much for contacting us a while back about the HPV16E6 + HPV18 E6 antibody ab70.

I sincerely apologize for the extended delay in getting this information for you, and I appreciate your patience while I have been in touch with th...

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About your questions and suggestions for ab70 antibody, the customer
answers are below:
She did the assay again, changing her protocol as suggest, however the
unspecific staining remains.
answers:
1- Her cells are monolayer, an...

Read More

Thank you for your reply.

As we have not previously tested this antibody in ICC/IF, we could not guarantee that it would work for this application, as under our Abpromise we only guarantee it to work in WB, IP& IHC-P.

However, I ...

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I am currently doing my MSC project on HPV in head and neck cancer and happens to remember you work with abcam. I can see that you have the Anti-HPV16 E6 + HPV18 E6 antibody [C1P5]. I would like to make a comparison of its sensitivity with p16ink4a if ...

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Thank you very much for your interest in http://www.abcam.com/ab70 (Anti-HPV16 E6 + HPV18 E6 antibody [C1P5])

To our knowledge,ab70 has not been tested with p16ink4a. Therefore, I can offer a discount off a future purchase if you buy ab70 now,...

Read More

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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