Anti-HPV16 E2抗体[TVG 261] (ab17185)

概述

  • 产品名称
    Anti-HPV16 E2抗体[TVG 261]
    参阅全部 HPV16 E2 一抗
  • 描述
    小鼠单克隆抗体[TVG 261] to HPV16 E2
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    Reacts with HPV E2.
  • 免疫原

    Vaccinia-E2 followed by intravenous injection of the maltose binding protein MBP-E2 (generated from an expression vector in which the E2 ORF was cloned in frame with malE).

  • 表位
    aa2-17 - ETLCQRLNVCQDKILT.
  • 常规说明


    The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. E1 and E2 are proteins involved in the regulation of viral DNA replication. TVG 261 is useful for detection of protein in cervical lesions.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    IgG fraction
  • Primary antibody说明
    The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. E1 and E2 are proteins involved in the regulation of viral DNA replication. TVG 261 is useful for detection of protein in cervical lesions.
  • 克隆
    单克隆
  • 克隆编号
    TVG 261
  • 骨髓瘤
    NS0/1
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab17185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.

靶标

  • 别名
    • HPV16 regulatory protein E2 antibody
    • Human papilloma virus 16 E2 antibody
    • Human papilloma virus 16 regulatory protein E2 antibody
    • Human papillomavirus type 16 E2 antibody
    • Human papillomavirus type 16 regulatory protein E2 antibody
    • Regulatory protein E2 antibody
    see all

实验方案

Anti-HPV16 E2 antibody [TVG 261] (ab17185)参考文献

This product has been referenced in:
  • Harris L  et al. The Cellular DNA Helicase ChlR1 Regulates Chromatin and Nuclear Matrix Attachment of the Human Papillomavirus 16 E2 Protein and High-Copy-Number Viral Genome Establishment. J Virol 91:N/A (2017). WB . Read more (PubMed: 27795438) »
  • Sharma R & Palefsky JM Addition of a single E2 binding site to the human papillomavirus (HPV) type 16 long control region enhances killing of HPV positive cells via HPV E2 protein-regulated herpes simplex virus type 1 thymidine kinase-mediated suicide gene therapy. Hum Gene Ther 21:843-54 (2010). WB . Read more (PubMed: 20132050) »

See all 4 Publications for this product

Product Wall

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Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
300000 cells
Specification
293T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Aug 12 2010

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos)
Loading amount
500000 cells
Specification
Cos
Treatment
Transfected with MV11.HPV16E2
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Clare Davy

Verified customer

提交于 Sep 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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