Anti-HPV11 E2抗体[10E12] (ab100968)

概述

  • 产品名称Anti-HPV11 E2抗体[10E12]
  • 描述
    小鼠单克隆抗体[10E12] to HPV11 E2
  • 特异性Reacts in WB, and ELISA with HPV11 native E2 protein as well as recombinant E2 protein expressed in bacteria and Cos 7 cells.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Human Papillomavirus (HPV)
  • 免疫原

    Recombinant full length HPV11 E2 protein purified from bacteria.

  • 表位Mapped to amino acids 202-284
  • 阳性对照
    • U20S or Hela cells and lysates

性能

应用

Our Abpromise guarantee covers the use of ab100968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 42 kDa.
ELISA 1/32000.
ICC/IF Use at an assay dependent dilution.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 相关性Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
    • 细胞定位Host nucleus
    • 数据库链接
      • 别名
        • Human papillomaviruses 11 E2 antibody

      Anti-HPV11 E2 antibody [10E12] 图像

      • All lanes : Anti-HPV11 E2 antibody [10E12] (ab100968) at 1/1000 dilution

        Lane 1 : lysate of U20S cells transfected with plasmid expressing HPV11 E2 protein
        Lane 2 : lysate of plain U20S cells
        Lane 3 : lysate of plain HeLa cells
        Lane 4 : lysate of U20S cells transfected with plasmid expressing HPV11 E2 protein
        Lane 5 : marker


        Predicted band size : 42 kDa
      • Immunofluorescence (IF) analysis of HPV11 E2 protein in U2OS cells.
        IF was carried out with U2OS cell transfected with plasmid expressing HPV11 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E2 detected by ab100968. IF analysis of E2 protein shows similar result also in HeLa cells.

      Anti-HPV11 E2 antibody [10E12] (ab100968)参考文献

      ab100968 has not yet been referenced specifically in any publications.

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