概述

  • 产品名称Anti-HPRT抗体
    参阅全部 HPRT 一抗
  • 描述
    兔多克隆抗体to HPRT
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Gerbil, Chinese Hamster
  • 免疫原

    Synthetic peptide derived from residues 200 to the C-terminus of Human HPRT.

  • 阳性对照
    • This antibody gave a positive control in the following human whole cell lysates: HeLa (Human epithelial carcinoma cell line) A431 (Human epithelial carcinoma cell line) MCF-7 (Human breast adenocarcinoma cell line) HEK 293 (Human embryonic kidney cell line) This antibody gave a positive control in the following mouse lysates: NIH 3T3 (Mouse embryonic fibroblast cell line) whole cell MEF1 (Mouse embryonic fibroblast cell line) whole cell Brain (mouse) tissue

性能

应用

Our Abpromise guarantee covers the use of ab10479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 24 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
  • 通路Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.
  • 疾病相关Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS) [MIM:300322]. LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation.
    Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT) [MIM:300323]; also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia.
  • 序列相似性Belongs to the purine/pyrimidine phosphoribosyltransferase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HGPRT antibody
    • HGPRTase antibody
    • HPRT 1 antibody
    • HPRT_HUMAN antibody
    • HPRT1 antibody
    • Hypoxanthine guanine phosphoribosyltransferase antibody
    • Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody
    • Hypoxanthine phosphoribosyltransferase 1 antibody
    • Hypoxanthine-guanine phosphoribosyltransferase antibody
    see all

Anti-HPRT antibody 图像



  • Predicted band size : 24 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: HPRT1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: A431 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab10479 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab10479 was shown to specifically react with HPRT1 when HPRT1 knockout samples were used. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE.  Ab10479 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • ICC/IF image of ab10479 stained human HeLa cells. The cells were methanol fixed ( 5 min) and incubated with the antibody (ab10479, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).

  • All lanes : Anti-HPRT antibody (ab10479) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 whole cell lysate (ab7909)
    Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 whole cell lysate (ab7902)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • All lanes : Anti-HPRT antibody (ab10479) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 28 kDa (possible glycosylated form).
  • Image courtesy of Human Protein Atlas. ab10479 staining HPRT in human small intestine. Paraffin embedded human small intestine tissue was incubated with ab10479 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab10479 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa


    Exposure time : 1 minute

Anti-HPRT antibody (ab10479)参考文献

This product has been referenced in:
  • Luxenburg C  et al. Wdr1-mediated cell shape dynamics and cortical tension are essential for epidermal planar cell polarity. Nat Cell Biol 17:592-604 (2015). Mouse . Read more (PubMed: 25915128) »
  • Appel S  et al. CNN3 regulates trophoblast invasion and is upregulated by hypoxia in BeWo cells. PLoS One 9:e103216 (2014). WB . Read more (PubMed: 25050546) »

See all 13 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (4-20% Tris Glycin)
Sample Mouse Cell lysate - whole cell (beta cells)
Specification beta cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Hippocampus)
Loading amount 5 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse striatum and liver)
Loading amount 20 µg
Specification Mouse striatum and liver
Gel Running Conditions Reduced Denaturing (4-12% TG)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Laura Ling

Verified customer

提交于 Jul 31 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"