Anti-HP1 gamma (unmodified S93)抗体(ab79304)

概述

  • 产品名称Anti-HP1 gamma (unmodified S93)抗体
    参阅全部 HP1 gamma 一抗
  • 描述
    兔多克隆抗体to HP1 gamma (unmodified S93)
  • 特异性Detects endogenous levels of total HP1 gamma protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized non-phosphopeptide derived from human HP1 gamma around the phosphorylation site of serine 93 (RKSPLS).

  • 阳性对照
    • 293 nuclear lysate

性能

应用

Our Abpromise guarantee covers the use of ab79304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa.
ELISA 1/40000.

靶标

  • 功能Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • 序列相似性Contains 2 chromo domains.
  • 翻译后修饰Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
  • 细胞定位Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CBX 3 antibody
    • CBX3 antibody
    • CBX3_HUMAN antibody
    • Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
    • Chromobox homolog 3 antibody
    • Chromobox protein homolog 3 antibody
    • GAMMA antibody
    • HECH antibody
    • Heterochromatin like protein 1 antibody
    • Heterochromatin protein 1 homolog gamma antibody
    • Heterochromatin protein HP1 gamma antibody
    • HP1 gamma antibody
    • HP1 gamma homolog antibody
    • HP1Hs gamma antibody
    • Modifier 2 protein antibody
    see all

Anti-HP1 gamma (unmodified S93) antibody 图像

  • All lanes : Anti-HP1 gamma (unmodified S93) antibody (ab79304) at 1/500 dilution

    Lane 1 : Extracts from 293 cells with no immunizing peptide
    Lane 2 : Extracts from 293 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Observed band size : 21 kDa (why is the actual band size different from the predicted?)

Anti-HP1 gamma (unmodified S93) antibody (ab79304)参考文献

ab79304 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Raw 264.7)
Loading amount 40 µg
Specification Raw 264.7
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (CBX3 recombinant protein)
Loading amount 0.1 µg
Specification CBX3 recombinant protein
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

James Thorne

Verified customer

提交于 Jul 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"