The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: Use at 2-5µg/mg of lysate.
WB: Use at a concentration of 1 µg/ml for enriched sources (e.g.
Immunoprecipitates). Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). Not recommended for crude preparations.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
序列相似性Contains 2 chromo domains.
翻译后修饰Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
细胞定位Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
Detection of HP1 gamma by Western Blot of Immunprecipitate.
ab72042 at 1µg/ml staining HP1 gamma in HeLa whole cell lysate immunoprecipitated using ab72042 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
Anti-HP1 gamma antibody (ab72042)参考文献
has not yet been referenced specifically in any publications.