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Our Abpromise guarantee covers the use of ab107100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2. Predicted molecular weight: 22 kDa.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HP1 alpha knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab107100 observed at 28 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab107100 was shown to specifically react with HP1 alpha when HP1 alpha knockout samples were used. Wild-type and HP1 alpha knockout samples were subjected to SDS-PAGE. ab107100 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rat IgG (H + L) (IRDye® 800CW) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
ab107100 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"