Anti-HP1 alpha抗体- Heterochromatin marker (ab9057)

概述

  • 产品名称Anti-HP1 alpha抗体- Heterochromatin marker
    参阅全部 HP1 alpha 一抗
  • 描述
    兔多克隆抗体to HP1 alpha - Heterochromatin marker
  • 经测试应用适用于: ICC/IF, IHC-P, WB, ICCmore details
    不适用于: ChIP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HP1 alpha.

    (Peptide available as ab4718.)

  • 阳性对照
    • In Western Blot, this antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; SHSY5Y.
  • 常规说明

    This antibody was thought to work in plants, but the localisation patterns, though specific, were not co-localised with methylated Histone H3 K9 as is found in other species.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab9057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).Can be blocked with HP1 alpha peptide (ab4718).
ICC 1/500.
  • 应用说明Is unsuitable for ChIP.
  • 靶标

    • 功能Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
    • 序列相似性Contains 2 chromo domains.
    • 翻译后修饰Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
      Ubiquitinated.
    • 细胞定位Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Antigen p25 antibody
      • CBX5 antibody
      • CBX5_HUMAN antibody
      • CG8409 antibody
      • Chromobox 5 antibody
      • Chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody
      • Chromobox homolog 5 antibody
      • Chromobox protein homolog 5 antibody
      • Epididymis luminal protein 25 antibody
      • HEL25 antibody
      • Heterochromatin protein 1 alpha antibody
      • Heterochromatin protein 1 antibody
      • Heterochromatin protein 1 homolog alpha antibody
      • HP1 alpha antibody
      • HP1 alpha homolog antibody
      • HP1 antibody
      • HP1A antibody
      • HP1Hs alpha antibody
      • Su(var)205 antibody
      see all

    Anti-HP1 alpha antibody - Heterochromatin marker 图像

    • All lanes : Anti-HP1 alpha antibody - Heterochromatin marker (ab9057) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) at 1/50000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 22 kDa
      Observed band size : 26 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 36 kDa,47 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 8 minutes

      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab9057 overnight at 4°C. Antibody binding was detected using an anti-rabbit IgG VHH single domain antibody (ab191866)conjugated to HRP, and visualised using ECL development solution ab133406.



    • Predicted band size : 22 kDa

      Rabbit polyclonal to HP1 alpha (ab9057). 20 ug HeLa cell lysate per lane.

      Lane 1 : ab9057 at 1/1000
      Lane 2 : ab9057 at 1/1000 + 1 ug blocking peptide (ab4718)

      Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721) at 1/2000.

    • Detection of ab9057 in a blood sample. This result is compatible with other results obtained with other HP1 antibodies (Minc et al., 2000 Cytogenet Cell Genet 90:279 –284). (Manuela Silva, personal communication).

    • ab9057 staining HP1 alpha in human A549 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.25% Triton and then blocked using 3% BSA for 1 hour at 18°C. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/800 dilution. Nuclei stained with DAPI (blue).

      See Abreview


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 22 kDa

    Anti-HP1 alpha antibody - Heterochromatin marker (ab9057)参考文献

    This product has been referenced in:
    • Perner J  et al. Inference of interactions between chromatin modifiers and histone modifications: from ChIP-Seq data to chromatin-signaling. Nucleic Acids Res 42:13689-95 (2014). IP . Read more (PubMed: 25414326) »
    • Deutsch MJ  et al. The latent origin of replication of Epstein-Barr virus directs viral genomes to active regions of the nucleus. J Virol 84:2533-46 (2010). ICC/IF ; Human . Read more (PubMed: 20032186) »

    See all 9 Publications for this product

    Product Wall

    Thank you for contacting us. The isotype of this rabbit polyclonal antibody is IgG. I have updated the datasheet accordingly. Thank you for pointing this out to us. I hope this information is helpful to you. Please do not hesitate to contact us...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (A549 - lung)
    Specification A549 - lung
    Fixative Paraformaldehyde
    Permeabilization Yes - Triton 0.25%
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 25 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Colorectal Cancer Cells)
    Loading amount 200 µg
    Specification Colorectal Cancer Cells
    Gel Running Conditions Reduced Denaturing (12% Gel)
    Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 04 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Human Cancer Distal Colon)
    Specification Human Cancer Distal Colon
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO Target Retrieval Solution
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 07 2008

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (human embryonic kidney cell)
    Specification human embryonic kidney cell
    Fixative Paraformaldehyde
    Blocking step 2% BSA or 10 % donkey serum or both as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2-10%
    Username

    Mr. Tuempong Wongtawan

    Verified customer

    提交于 Jan 03 2007

    Thank you for getting back to me. I appreciate the steps that you have taken to optimise this antibody. I am disappointed that there was not a significant improvement in the results that you obtained. I look forward to receiving your results fr...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (fibroblast)
    Specification fibroblast
    Fixative Formaldehyde
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2%
    Username

    Mr. Tuempong Wongtawan

    Verified customer

    提交于 Nov 17 2006

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I appreciate the image that you have sent me in order to support your enquiry. Rabbit polyclonal to HP1 alpha has been applied and is guarantee...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. HP1 alpha antibody - Heterochromatin marker (ab9057) is a good selling antibody that we produced in house. I would very much appreciate it if ...

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    We recommend that you block the membrane in 5% w/v BSA (fractionV) NOT MILK (milk contains casein which is a phosphoprotein; This is why it causes high background because the phospho-specific antibody detects the casein present in the milk). If you ...

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