Anti-HP1 alpha抗体- ChIP Grade (ab77256)

概述

  • 产品名称
    Anti-HP1 alpha抗体- ChIP Grade
    参阅全部 HP1 alpha 一抗
  • 描述
    山羊多克隆抗体to HP1 alpha - ChIP Grade
  • 经测试应用
    适用于: ICC/IF, IP, IHC-P, ChIP, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Rhesus monkey, Chinese hamster
  • 免疫原

    Synthetic peptide:

    C-NKRKSNFSNSADDIK

    , corresponding to internal sequence amino acids 88-102 of Human HP1 alpha (NP_036249.1).

  • 阳性对照
    • Human fibroblast cell lines A431 and HeLa cell lysates. Human uterus tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab77256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 2 - 3 µg/ml.
ChIP Use at an assay dependent dilution. PubMed: 20663865
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

靶标

  • 功能
    Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • 序列相似性
    Contains 2 chromo domains.
  • 翻译后修饰
    Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
    Ubiquitinated.
  • 细胞定位
    Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen p25 antibody
    • CBX5 antibody
    • CBX5_HUMAN antibody
    • CG8409 antibody
    • Chromobox 5 antibody
    • Chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody
    • Chromobox homolog 5 antibody
    • Chromobox protein homolog 5 antibody
    • Epididymis luminal protein 25 antibody
    • HEL25 antibody
    • Heterochromatin protein 1 alpha antibody
    • Heterochromatin protein 1 antibody
    • Heterochromatin protein 1 homolog alpha antibody
    • HP1 alpha antibody
    • HP1 alpha homolog antibody
    • HP1 antibody
    • HP1A antibody
    • HP1Hs alpha antibody
    • Su(var)205 antibody
    see all

Anti-HP1 alpha antibody - ChIP Grade 图像

  • All lanes : Anti-HP1 alpha antibody - ChIP Grade (ab77256) at 0.3 µg/ml

    Lane 1 : A431 cell line lysates in RIPA buffer
    Lane 2 : HeLa cell line lysates in RIPA buffer
    Lane 3 : NIH3T3 cell line lysates in RIPA buffer

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 22 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • ab77256 at 2.5µg/ml staining HP1 alpha in human uterus tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

  • ab77256 staining HP1 alpha in Mouse 3T3 fibroblasts by Immunocytochemistry/ Immunoflourescence. Cells were PFA-fixed and permeabilized in 0.2% Triton X-100 in PBS. The primary antibody was diluted 1/100 in PBS + Tween 1:1000 and incubated with the sample for 30 minutes. The secondary antibody was Cy2®-conjugated Donkey anti-Goat polyclonal, diluted 1/100.

    See Abreview

  • Anti-HP1 alpha antibody - ChIP Grade (ab77256) at 0.03 µg/ml + HeLa lysate in RIPA buffer at 35 µg

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab77256 (2µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-HP1 alpha antibody - ChIP Grade (ab77256)参考文献

This product has been referenced in:
  • Yang G  et al. The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. Nat Commun 8:14941 (2017). ChIP . Read more (PubMed: 28361889) »
  • Magaraki A  et al. Silencing markers are retained on pericentric heterochromatin during murine primordial germ cell development. Epigenetics Chromatin 10:11 (2017). IF ; Mouse . Read more (PubMed: 28293300) »

See all 16 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing (4-20% Gradiant)
Loading amount
20 µg
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 25 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Epithelial Cells)
Permeabilization
Yes - 0.2% TritonX-100
Specification
Epithelial Cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (B Cells)
Negative control
Isotype IgG
Specification
B Cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control
Specific Gene Promoter
Username

Abcam user community

Verified customer

提交于 Nov 28 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Ahmar Aziz

Verified customer

提交于 May 03 2016

Application
Western blot
Sample
Rat Cell lysate - whole cell (Epithelial cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
75 µg
Specification
Epithelial cells
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Nov 11 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (HeLa, RPE-1)
Specification
HeLa, RPE-1
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jul 08 2013

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (HEK-293)
Total protein in input
1000 µg
Specification
HEK-293
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

提交于 Mar 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (HEK-293)
Loading amount
200000 cells
Specification
HEK-293
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Benny Garfinkel

Verified customer

提交于 Feb 03 2011

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