概述

  • 产品名称
  • 描述
    兔多克隆抗体to HOXD9
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 144-193 (GPSPGPSGPA NGRHYGIKPE TRAAPAPATA ASTTSSSSTS LSSSSKRTEC) of Human HOXD9 (NP_055028).

  • 阳性对照
    • Human fetal spleen lysate

性能

应用

Our Abpromise guarantee covers the use of ab90260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration. PubMed: 24651450

靶标

图片

  • Anti-HOXD9 antibody (ab90260) at 1 µg/ml + Human fetal spleen lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa

文献

This product has been referenced in:
  • Toshner M  et al. Transcript analysis reveals a specific HOX signature associated with positional identity of human endothelial cells. PLoS One 9:e91334 (2014). IHC-P ; Human . Read more (PubMed: 24651450) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Rat Tissue lysate - whole (brain)
Specification
brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 23 2014

Thank you for the details of your samples and protocol. This is strong evidence that the antibody cannot detect mouse HOXD9. The "predicted" reactivity with the mouse protein is based on homology with the immunogen amino acid sequence, but the shared i...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册