Anti-HOXD10抗体(ab76897)

概述

  • 产品名称Anti-HOXD10抗体
    参阅全部 HOXD10 一抗
  • 描述
    兔多克隆抗体to HOXD10
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Cow, Chimpanzee, Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human HOXD10.

    (Peptide available as ab90993.)

  • 阳性对照
    • This antibody gave a positive signal in JEG3 whole cell lysate and in the following tissue lysates: Human ovary; Human testis; Human prostate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab76897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-Fr 1/16000.

靶标

  • 功能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 组织特异性Strongly expressed in the adult male and female urogenital tracts.
  • 疾病相关Defects in HOXD10 are a cause of congenital vertical talus (CVT) [MIM:192950]; also known as rocker-bottom foot deformity or congenital convex pes valgus. CVT is a dislocation of the talonavicular joint, with rigid dorsal dislocation of the navicular over the neck of the talus. This condition is usually associated with multiple other congenital deformities and only rarely is an isolated deformity.
  • 序列相似性Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段Expressed in the developing limb buds.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI385591 antibody
    • AI874987 antibody
    • Homeo box 4D antibody
    • Homeo box D10 antibody
    • Homeobox D10 antibody
    • Homeobox protein Hox-4D antibody
    • Homeobox protein Hox-4E antibody
    • Homeobox protein Hox-D10 antibody
    • Hox 4.4 antibody
    • Hox 4.5 antibody
    • Hox 4D antibody
    • Hox 4E antibody
    • Hox 5.3 antibody
    • HOX4D antibody
    • HOX4E antibody
    • HOXD10 antibody
    • HXD10 antibody
    • HXD10_HUMAN antibody
    • OTTMUSP00000017845 antibody
    • RP23-313J15.9 antibody
    see all

Anti-HOXD10 antibody 图像

  • All lanes : Anti-HOXD10 antibody (ab76897) at 1 µg/ml

    Lane 1 : Human ovary tissue lysate - total protein (ab30222)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human prostate tissue lysate - total protein (ab30304)
    Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 146 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ab76897 staining HOXD10 in Mouse spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/16000 in PBS + 0.1% Triton + 0.1% BSA) for 18 hours at 4°C. A Cy3®-conjugated Donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.

    See Abreview

Anti-HOXD10 antibody (ab76897)参考文献

ab76897 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spinal Cord)
Specification Spinal Cord
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Jul 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"