概述

  • 产品名称Anti-HOXC9抗体[HOXCA6E6]
    参阅全部 HOXC9 一抗
  • 描述
    小鼠单克隆抗体[HOXCA6E6] to HOXC9
  • 特异性This antibody reacts with HOXC9.
  • 经测试应用适用于: ICC/IF, ICC, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    HOXC9 recombinant fragment (Human), containing 50-200 aa

  • 阳性对照
    • Hela cells and HT-1080 whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab50839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
ICC 1/5.
WB 1/100. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

靶标

  • 功能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 序列相似性Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homeo box C9 antibody
    • Homeobox C9 antibody
    • Homeobox protein Hox C9 antibody
    • Homeobox protein Hox-3B antibody
    • Homeobox protein Hox-C9 antibody
    • Hox 3B antibody
    • HOX3 antibody
    • HOX3B antibody
    • Hoxc9 antibody
    • HXC9_HUMAN antibody
    see all

Anti-HOXC9 antibody [HOXCA6E6] 图像

  • Anti-HOXC9 antibody [HOXCA6E6] (ab50839) at 1/100 dilution + HT-1080 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • ab50839 at a 1/5 dilution staining HOXC9 in Hela cells using Alexa Fluor® 488 Goat Anti-mouse IgG at a 1/200 dilution.

Anti-HOXC9 antibody [HOXCA6E6] (ab50839)参考文献

This product has been referenced in:
  • Stoll SJ  et al. HOXC9 regulates formation of parachordal lymphangioplasts and the thoracic duct in zebrafish via stabilin 2. PLoS One 8:e58311 (2013). Read more (PubMed: 23484014) »
  • Stoll SJ  et al. The Transcription Factor HOXC9 Regulates Endothelial Cell Quiescence and Vascular Morphogenesis in Zebrafish via Inhibition of Interleukin 8. Circ Res 108:1367-77 (2011). Read more (PubMed: 21493894) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample Human Cell (embryonic stem cells)
Specification embryonic stem cells
Permeabilization Yes - PBST
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Aug 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Neuroblastoma cell line)
Loading amount 40 µg
Specification Neuroblastoma cell line
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Mar 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"