概述

  • 产品名称Anti-HOXC8抗体
    参阅全部 HOXC8 一抗
  • 描述
    兔多克隆抗体to HOXC8
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Sheep, Chicken, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HOXC8.

    (Peptide available as ab101082.)

  • 阳性对照
    • This antibody gave a positive signal in Mouse Spinal Cord and Mouse Brain Tissue Lysates.

性能

应用

Our Abpromise guarantee covers the use of ab79690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

靶标

  • 功能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 序列相似性Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homeo box 3A antibody
    • Homeo box 8 antibody
    • Homeobox 3A antibody
    • Homeobox C8 antibody
    • Homeobox protein Hox C8 antibody
    • Homeobox protein Hox-3A antibody
    • Homeobox protein Hox-C8 antibody
    • Homeobox protein HoxC8 antibody
    • Homeobox protein R4 antibody
    • Homolog of mouse Hox 3.1 antibody
    • HOX 3 antibody
    • Hox 3.1 mouse homolog of antibody
    • HOX 3A antibody
    • HOX C8 antibody
    • Hox-3.1 antibody
    • Hox-3A antibody
    • HOX3 antibody
    • HOX3A antibody
    • HOXC 8 antibody
    • Hoxc-8 antibody
    • HOXC8 antibody
    • HXC8_HUMAN antibody
    • M31 antibody
    see all

Anti-HOXC8 antibody 图像

  • All lanes : Anti-HOXC8 antibody (ab79690) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa


    Exposure time : 16 minutes
  • ICC/IF image of ab79690 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79690, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HOXC8 antibody (ab79690)参考文献

ab79690 has not yet been referenced specifically in any publications.

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