概述

  • 产品名称Anti-HOXB9抗体
    参阅全部 HOXB9 一抗
  • 描述
    兔多克隆抗体to HOXB9
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: SLPSVYHPYI QPQGVPPAES RYLRTWLEPA PRGEAAPGQG QAAVKAEPLL, corresponding to amino acids 73-122 of Human HOXB9

  • 阳性对照
    • HepG2 cell lysate, human heart tissue and human lung tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab66765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1.25 µg/ml. Detects a band of approximately 28, 32, 85 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 序列相似性Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段Expressed in whole embryos and fetuses at 5-9 weeks from conception.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homeo box 2E antibody
    • Homeo box B9 antibody
    • Homeobox B9 antibody
    • Homeobox protein Hox B9 antibody
    • Homeobox protein Hox-2.5 antibody
    • Homeobox protein Hox-2E antibody
    • Homeobox protein Hox-B9 antibody
    • Hox 2.5 antibody
    • Hox 2E antibody
    • HOX2 antibody
    • HOX2E antibody
    • Hoxb9 antibody
    • HXB9_HUMAN antibody
    • OTTHUMP00000216946 antibody
    • OTTHUMP00000243325 antibody
    see all

Anti-HOXB9 antibody 图像

  • Anti-HOXB9 antibody (ab66765) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 30 kDa,85 kDa. We are unsure as to the identity of these extra bands.
    There are also three other bands < 23kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling HOXB9 with ab66765 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling HOXB9 with ab66765 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.

Anti-HOXB9 antibody (ab66765)参考文献

This product has been referenced in:
  • Wu SY  et al. A miR-192-EGR1-HOXB9 regulatory network controls the angiogenic switch in cancer. Nat Commun 7:11169 (2016). WB, IHC-P ; Human . Read more (PubMed: 27041221) »

See 1 Publication for this product

Product Wall

Thank you for your reply and for your interest in ab66765. I will have the blocking peptide added to the catalog, and I'll let you know the catalog number once this is completed. Since ab66765 has not been tested in bovine or murine samples, if you wou...

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Thank you for contacting us with your questions. We have not tested this antibody yet in bovine or murine tissue, however the immunogen is 100% homologous to the bovine sequence so it will most likely work. The immunogen is only 78% homologous to mouse...

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Thank you for contacting us and please accept all our apologies for this error and delay. Indeed, we had a confusion in our notes in regards to the reconstitution of this antibody. I can confirm now, that the vial you have received has been rec...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Oct 21 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"