产品名称 Anti-HOXB8抗体 参阅全部 HOXB8 一抗
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Mouse, Rat, Dog, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster
Synthetic peptide corresponding to Human HOXB8 aa 200 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P17481
This antibody gave a positive signal in both Human Fetal Liver and Human Fetal Brain tissue lysates.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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纯度 Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).
功能 Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
序列相似性 Belongs to the Antp homeobox family. Contains 1 homeobox DNA-binding domain.
发展阶段 Expressed in whole embryos and fetuses at 5-9 weeks from conception.
Information by UniProt
Homeobox B8 antibody
Homeobox protein Hox B8 antibody
Homeobox protein Hox-2.4 antibody
Anti-HOXB8 antibody 图像
Western blot - Anti-HOXB8 antibody (ab125727)
All lanes : Anti-HOXB8 antibody (ab125727) at 1 µg/ml Lane 1 : Fetal Liver (Human) Normal Tissue Lysate Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue Lysates/proteins at 25 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) ( ab97051) at 1/10000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 28 kDa Observed band size : 32 kDa ( why is the actual band size different from the predicted?) Additional bands at : 48 kDa (possible non-specific binding),52 kDa (possible non-specific binding). Exposure time : 16 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab125727 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Anti-HOXB8 antibody (ab125727)参考文献
has not yet been referenced specifically in any publications.
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