Mouse, Rat, Dog, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
Synthetic peptide corresponding to Human HOXB8 aa 200 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P17481
This antibody gave a positive signal in both Human Fetal Liver and Human Fetal Brain tissue lysates.
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Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Belongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain.
Expressed in whole embryos and fetuses at 5-9 weeks from conception.
Information by UniProt
Homeobox B8 antibody
Homeobox protein Hox B8 antibody
Homeobox protein Hox-2.4 antibody
Western blot - Anti-HOXB8 antibody (ab125727)
All lanes :
Anti-HOXB8 antibody (ab125727) at 1 µg/ml
Lane 1 :
Fetal Liver (Human) Normal Tissue Lysate
Lane 2 :
Brain (Human) Tissue Lysate - fetal normal tissue
Lysates/proteins at 25 µg per lane.
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
32 kDa (
why is the actual band size different from the predicted?
Additional bands at :
48 kDa (possible non-specific binding),52 kDa (possible non-specific binding).
Exposure time :
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab125727 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
has not yet been referenced specifically in any publications.
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