概述

  • 产品名称Anti-HOXB8抗体
    参阅全部 HOXB8 一抗
  • 描述
    兔多克隆抗体to HOXB8
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide corresponding to Human HOXB8 aa 200 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P17481

  • 阳性对照
    • This antibody gave a positive signal in both Human Fetal Liver and Human Fetal Brain tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab125727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).

靶标

  • 功能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 序列相似性Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段Expressed in whole embryos and fetuses at 5-9 weeks from conception.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homeobox B8 antibody
    • Homeobox protein Hox B8 antibody
    • Homeobox protein Hox-2.4 antibody
    • Homeobox protein Hox-2D antibody
    • Homeobox protein Hox-B8 antibody
    • Homeobox protein HoxB8 antibody
    • HOX 2 antibody
    • Hox 2.4 antibody
    • HOX 2D antibody
    • HOX B8 antibody
    • HOX2 antibody
    • Hox2.4 antibody
    • HOX2D antibody
    • Hoxb8 antibody
    • HXB8_HUMAN antibody
    see all

Anti-HOXB8 antibody 图像

  • All lanes : Anti-HOXB8 antibody (ab125727) at 1 µg/ml

    Lane 1 : Fetal Liver (Human) Normal Tissue Lysate
    Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa (possible non-specific binding),52 kDa (possible non-specific binding).

    Exposure time : 16 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab125727 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-HOXB8 antibody (ab125727)参考文献

ab125727 has not yet been referenced specifically in any publications.

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