Anti-HOXB5抗体[EP2827] (ab109375)
Key features and details
- Rabbit monoclonal [EP2827] to HOXB5
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-HOXB5抗体[EP2827]
参阅全部 HOXB5 一抗 -
描述
兔单克隆抗体[EP2827] to HOXB5 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal lung and Human fetal kidney lysates
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP2827 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109375于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
靶标
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功能
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
组织特异性
Spinal cord. -
序列相似性
Belongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain. -
发展阶段
Embryo. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3215 Human
- Entrez Gene: 15413 Mouse
- Entrez Gene: 497987 Rat
- Omim: 142960 Human
- SwissProt: P09067 Human
- SwissProt: P09079 Mouse
- Unigene: 654456 Human
- Unigene: 207 Mouse
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别名
- HGNC:5116 antibody
- HHO.C10 antibody
- Homeo box 2A antibody
see all
图片
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All lanes : Anti-HOXB5 antibody [EP2827] (ab109375) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg/ml per lane.
Predicted band size: 29 kDa -
ab109375 staining the HOXB5 in U-87 MG (Human glioblastoma-astrocytoma epithelial cell) by intracellular flow cytometry. The sample was incubated with the primary antibody at 1/80 (Pink) for 30 minutes at 4°C. A FITC-conjugated Goat anti-rabbit IgG (1/150) was used as the secondary antibody.
Rabbit monoclonal IgG was used as an Isotype control (Green)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab109375 被引用在 5 文献中.
- Li J & Gao S HOXB5-activated ANGPT2 promotes the proliferation, migration, invasion and angiogenic effect of esophageal cancer cells via activating ERK/AKT signaling pathway. Exp Ther Med 24:585 (2022). PubMed: 35949323
- Zhao J et al. The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulates the proliferation of glioma stem cells via the IL6-mediated JAK2/STAT3 signaling pathway. J Exp Clin Cancer Res 40:134 (2021). PubMed: 33858489
- Chen M et al. The Prognostic Value and Function of HOXB5 in Acute Myeloid Leukemia. Front Genet 12:678368 (2021). PubMed: 34421991
- Lin C et al. Long non-coding RNA PRNCR1 exerts oncogenic effects in tongue squamous cell carcinoma in vitro and in vivo by sponging microRNA-944 and thereby increasing HOXB5 expression. Int J Mol Med 46:119-130 (2020). PubMed: 32319550
- Wang Y et al. CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathway. J Exp Clin Cancer Res 39:169 (2020). PubMed: 32847606