Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee
Synthetic peptide corresponding to a region within N terminal amino acids 35-84 (DPAAMHTGSY GYNYNGMDLS VNRSSASSSH FGAVGESSRA FPAPAQEPRF) of Human HOXB5 (NP_002138).
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Belongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain.
Information by UniProt
- HGNC:5116 antibody
- HHO.C10 antibody
- Homeo box 2A antibody
Western blot - Anti-HOXB5 antibody (ab83025)
Anti-HOXB5 antibody (ab83025) at 1 µg/ml (5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
29 kDaObserved band size:
31 kDa (why is the actual band size different from the predicted?
)Additional bands at:
50 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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