RabMAb

Anti-HOXB4抗体[EP1919Y] (ab76093)

概述

  • 产品名称Anti-HOXB4抗体[EP1919Y]
    参阅全部 HOXB4 一抗
  • 描述
    兔单克隆抗体[EP1919Y] to HOXB4
  • 经测试应用适用于: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in the middle region of human HOXB4.

  • 阳性对照
    • K562 cell lysate, human placental tissue.
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab76093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IP 1/40.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 序列相似性Belongs to the Antp homeobox family. Deformed subfamily.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段Expressed in whole embryos and fetuses at 5-9 weeks from conception.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homeo box 2F antibody
    • Homeo box B4 antibody
    • Homeobox 2F antibody
    • Homeobox B4 antibody
    • Homeobox protein Hox B4 antibody
    • Homeobox protein Hox-2.6 antibody
    • Homeobox protein Hox-2F antibody
    • Homeobox protein Hox-B4 antibody
    • Homeobox protein HoxB4 antibody
    • HOX 2 antibody
    • Hox 2.6 antibody
    • Hox 2F antibody
    • HOX B4 antibody
    • HOX2 antibody
    • Hox2.6 antibody
    • Hox2F antibody
    • HOXB 4 antibody
    • hoxb4 antibody
    • HXB4_HUMAN antibody
    see all

Anti-HOXB4 antibody [EP1919Y] 图像

  • Anti-HOXB4 antibody [EP1919Y] (ab76093) at 1/500 dilution + K562 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 28 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human placental tissue, using ab76093, at 1/100 dilution.
  • Overlay histogram showing K562 cells stained with ab76093 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76093, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-HOXB4 antibody [EP1919Y] (ab76093)参考文献

ab76093 has not yet been referenced specifically in any publications.

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