概述

  • 产品名称
  • 描述
    兔多克隆抗体to Hoxb3
  • 经测试应用
    适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide within residues: GPSKSGPPKC GPGTNSTLTK QIFPWMKESR QTSKLKNNSP GTAEGCGGGG , corresponding to N terminal amino acids 108-157 of Human Hoxb3 (NP_002137)

  • 阳性对照
    • HepG2 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 25745994
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 功能
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 序列相似性
    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段
    Expressed in whole embryos and fetuses at 5-9 weeks from conception.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homeo box 2G antibody
    • Homeo box B3 antibody
    • Homeobox B3 antibody
    • Homeobox protein Hox-2.7 antibody
    • Homeobox protein Hox-2G antibody
    • Homeobox protein Hox-B3 antibody
    • Homeobox protein HoxB3 antibody
    • Hox 2.7 antibody
    • HOX 2G antibody
    • HOX2 antibody
    • Hox2.7 antibody
    • HOX2G antibody
    • HOXB3 antibody
    • HXB3_HUMAN antibody
    see all

图片

  • Anti-Hoxb3 antibody (ab83404) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa

实验方案

文献

This product has been referenced in:
  • Jiang Y  et al. Repression of Hox genes by LMP1 in nasopharyngeal carcinoma and modulation of glycolytic pathway genes by HoxC8. Oncogene 34:6079-91 (2015). IHC-P ; Human . Read more (PubMed: 25745994) »

See 1 Publication for this product

客户评价及客户问答

I have processed your request to receive a refund for ab83404 which did not work in western blot. Your credit note number is ******.

Thank you for your reply and for providing the order details. ab843404 is covered under our Abpromsie and so is guaranteed to work in western blotting on human samples. As it is clearly not working as promised, then I can either send you a replacement ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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