概述

  • 产品名称
  • 描述
    兔多克隆抗体to Hoxb3
  • 特异性
    A region of Human HOXB3
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (SCMRPGLAPE PLSAPPGSPP PSAAPTSATS NSSNGGGPSK SGPPKCGPGT) of human Hoxb3 (NP_002137).

  • 阳性对照
    • Fetal liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab82945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

靶标

  • 功能
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 序列相似性
    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段
    Expressed in whole embryos and fetuses at 5-9 weeks from conception.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homeo box 2G antibody
    • Homeo box B3 antibody
    • Homeobox B3 antibody
    • Homeobox protein Hox-2.7 antibody
    • Homeobox protein Hox-2G antibody
    • Homeobox protein Hox-B3 antibody
    • Homeobox protein HoxB3 antibody
    • Hox 2.7 antibody
    • HOX 2G antibody
    • HOX2 antibody
    • Hox2.7 antibody
    • HOX2G antibody
    • HOXB3 antibody
    • HXB3_HUMAN antibody
    see all

Anti-Hoxb3 antibody 图像

  • Anti-Hoxb3 antibody (ab82945) at 1 µg/ml (diluted in 5% skim milk / PBS buffer) + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-Hoxb3 antibody (ab82945)参考文献

ab82945 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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