概述

  • 产品名称Anti-HOXB13抗体
    参阅全部 HOXB13 一抗
  • 描述
    兔多克隆抗体to HOXB13
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Pufferfish (Fugu), Zebrafish, Japanese ricefish
  • 免疫原

    Synthetic peptide:

    EREYAANKFITKDKRRKI

    , corresponding to amino acids 232 - 249 of Human HOXB13

  • 阳性对照
    • HeLa cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab28575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 24189220
WB 1/1000 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
ELISA 1/100 - 1/1000.

靶标

Anti-HOXB13 antibody 图像

  • All lanes : Anti-HOXB13 antibody (ab28575) at 1/5000 dilution

    Lane 1 : Hela cell lysate with no immunizing peptide
    Lane 2 : Hela cell lysate with immunizing peptide

    Lysates/proteins at 20 µg/ml per lane.


    Predicted band size : 32 kDa
    Observed band size : 32 kDa

Anti-HOXB13 antibody (ab28575)参考文献

This product has been referenced in:
  • Cantile M  et al. Aberrant expression of posterior HOX genes in well differentiated histotypes of thyroid cancers. Int J Mol Sci 14:21727-40 (2013). IHC-P ; Human . Read more (PubMed: 24189220) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (prostate tumour derived line PC3)
Loading amount 20 µg
Specification prostate tumour derived line PC3
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
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提交于 Jan 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"