概述

  • 产品名称Anti-HOXA9抗体
    参阅全部 HOXA9 一抗
  • 描述
    兔多克隆抗体to HOXA9
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 223-272 (KEFLFNMYLT RDRRYEVARL LNLTERQVKI WFQNRRMKMK KINKDRAKDE) of Human HOXA9 (NP_689952).

  • 阳性对照
    • 721_B cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多���隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 疾病相关Note=A chromosomal aberration involving HOXA9 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with NUP98.
    Note=A chromosomal aberration involving HOXA9 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with MSI2.
  • 序列相似性Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 形式HOXA9 is a transcription factor with a central role in both haemopoiesis and leukaemia. High levels of HOXA9 expression in haemopoietic cells is a characteristic feature of acute myeloid leukaemia (AML), and may be sufficient to cause this disease. Overexpression of Hoxa 9 markedly expands hematopoietic stem cells.
  • 别名
    • ABD-B antibody
    • Abd-B, drosophila, homolog of antibody
    • D6a9 antibody
    • Homeobox 1G antibody
    • Homeobox A9 antibody
    • Homeobox protein Hox-1.7 antibody
    • Homeobox protein Hox-1G antibody
    • Homeobox protein Hox-A9 antibody
    • Homeodomain protein HOXA9 antibody
    • Hox-1.7 antibody
    • Hox-1.7, mouse, homolog of antibody
    • HOX1 antibody
    • Hox1.7 antibody
    • Hox1G antibody
    • Hox1r5 antibody
    • Hoxa-9 antibody
    • Hoxa7 antibody
    • HOXA9 antibody
    • HOXA9/MSI2 fusion gene, included antibody
    • HOXA9/NUP98 fusion gene, included antibody
    • HXA9_HUMAN antibody
    • MGC1934 antibody
    see all

Anti-HOXA9 antibody 图像

  • Anti-HOXA9 antibody (ab83480) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • ICC/IF image of ab83480 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83480, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HOXA9 antibody (ab83480)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (spinal cord)
Permeabilization Yes - 0.1% Tween
Specification spinal cord
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Paraformaldehyde
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Ms. Zahra Motahari

Verified customer

提交于 Aug 04 2015

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees ...

Read More

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"