Anti-HOXA5抗体(ab55239)

概述

  • 产品名称Anti-HOXA5抗体
    参阅全部 HOXA5 一抗
  • 描述
    小鼠单克隆抗体to HOXA5
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rabbit, Human
  • 免疫原

    Recombinant fragment: PAQPQIYPWM RKLHISHDNI GGPEGKRART AYTRYQTLEL EKEFHFNRYL TRRRRIEIAH ALCLSERQIK IWFQNRRMKW KKDNKLKSMS MAAAGGAFRP , corresponding to amino acids 171-271 of Human HOXA5

性能

应用

Our Abpromise guarantee covers the use of ab55239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Also binds to its own promoter. Binds specifically to the motif 5'-CYYNATTA[TG]Y-3'.
  • 序列相似性Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段Expressed during embryogenesis and in adult kidney.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homeo box 1C antibody
    • Homeo box A5 antibody
    • Homeobox A5 antibody
    • Homeobox protein Hox-1C antibody
    • Homeobox protein Hox-A5 antibody
    • Homeobox protein HOXA5 antibody
    • HOX 1 antibody
    • HOX 1.3 antibody
    • HOX 1C antibody
    • HOX1 antibody
    • HOX1.3 antibody
    • HOX1C antibody
    • HOXA5 antibody
    • HXA5_HUMAN antibody
    • MGC9376 antibody
    • OTTHUMP00000217259 antibody
    see all

Anti-HOXA5 antibody 图像

  • Anti-HOXA5 antibody (ab55239) + Human skeletal muscle

    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • Anti-HOXA5 antibody (ab55239) + Recombinant immunogen with 25 kDa tag.
  • All lanes : Anti-HOXA5 antibody (ab55239) at 1 µg/ml (Anti-HOXA5 Antibody )

    Lane 1 : HOXA5 Tranfected 293T cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat anti-mouse IgG-HRP

    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds
  • Overlay histogram showing HEK293 cells stained with ab55239 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55239, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HOXA5 antibody (ab55239)参考文献

ab55239 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (lung)
Loading amount 25 µg
Specification lung
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Mun-Suk Park

Verified customer

提交于 May 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (haematopoietic cell line)
Loading amount 40 µg
Specification haematopoietic cell line
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Nov 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"