重组
RabMAb

Anti-Hormone sensitive lipase (phospho S855)抗体[EPR2330] (ab76492)

概述

  • 产品名称
    Anti-Hormone sensitive lipase (phospho S855)抗体[EPR2330]
    参阅全部 Hormone sensitive lipase 一抗
  • 描述
    兔单克隆抗体[EPR2330] to Hormone sensitive lipase (phospho S855)
  • 特异性
    Detects HSL/LIPE only when phosphorylated on Serine 855
  • 经测试应用
    适用于: WBmore details
    不适用于: ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Phospho-specific peptide corresponding to residues surrounding Serine 855 of Human HSL/LIPE

  • 阳性对照
    • Human skeletal muscle lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

应用

Our Abpromise guarantee covers the use of ab76492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/1000 - 1/5000. Predicted molecular weight: 117 kDa (isoform 1) or 83 kDa (isoform 2).

    Is unsuitable for ICC/IF, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
    • 通路
      Glycerolipid metabolism; triacylglycerol degradation.
    • 序列相似性
      Belongs to the 'GDXG' lipolytic enzyme family.
    • 细胞定位
      Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Hormone sensitive lipase antibody
      • Hormone sensitive lipase testicular isoform antibody
      • Hormone-sensitive lipase antibody
      • HSL antibody
      • LHS antibody
      • Lipase hormone sensitive antibody
      • LIPE antibody
      • LIPS_HUMAN antibody
      see all

    图片

    • All lanes : Anti-Hormone sensitive lipase (phospho S855) antibody [EPR2330] (ab76492) at 1/5000 dilution

      Lane 1 : Human skeletal muscle lysate, untreated
      Lane 2 : Human skeletal muscle lysate, treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labeled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 117 , 83 kDa
      Observed band size : 83 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    ab76492 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Thank you for your inquiry.

    The Serine 855 of human HSL is equivalent to Serine 559 of mouse HSL, and this would recognize the Serine 559 of mouse HSL. The orthologous residue for isoform 2 of the rat HSL would be Serine 565, and this antibo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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