重组Anti-Hormone sensitive lipase/HSL (phospho S855)抗体[EPR2330] (ab76492)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2330] to Hormone sensitive lipase/HSL (phospho S855)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Hormone sensitive lipase/HSL (phospho S855)抗体[EPR2330]
参阅全部 Hormone sensitive lipase/HSL 一抗 -
描述
兔单克隆抗体[EPR2330] to Hormone sensitive lipase/HSL (phospho S855) -
宿主
Rabbit -
特异性
Detects HSL/LIPE only when phosphorylated on Serine 855 -
经测试应用
适用于: WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human skeletal muscle lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2330 -
同种型
IgG -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76492于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 117 , 83 kDa.
Predicted molecular weight: 117 kDa (isoform 1) or 83 kDa (isoform 2). |
说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 117 , 83 kDa. Predicted molecular weight: 117 kDa (isoform 1) or 83 kDa (isoform 2). |
靶标
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功能
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. -
通路
Glycerolipid metabolism; triacylglycerol degradation. -
序列相似性
Belongs to the 'GDXG' lipolytic enzyme family. -
细胞定位
Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation. - Information by UniProt
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数据库链接
- Entrez Gene: 3991 Human
- Entrez Gene: 16890 Mouse
- Omim: 151750 Human
- SwissProt: Q05469 Human
- SwissProt: Q6NSL7 Human
- SwissProt: P54310 Mouse
- Unigene: 656980 Human
- Unigene: 158548 Mouse
see all -
别名
- Hormone sensitive lipase antibody
- Hormone sensitive lipase testicular isoform antibody
- Hormone-sensitive lipase antibody
see all
图片
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All lanes : Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492) at 1/5000 dilution
Lane 1 : Human skeletal muscle lysate, untreated
Lane 2 : Human skeletal muscle lysate, treated with LP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/1000 dilution
Predicted band size: 117 , 83 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab76492 被引用在 1 文献中.
- Chadwick ML et al. Combined mTOR and MEK inhibition is an effective therapy in a novel mouse model for angiosarcoma. Oncotarget 9:24750-24765 (2018). PubMed: 29872503