The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 117 kDa).
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Western blot - HSL/LIPE (phospho S554) antibody (ab55228)
All lanes : Anti-Hormone sensitive lipase (phospho S855) antibody (ab55228) at 1/500 dilution
Lane 1 : extracts from HeLa cells treated
with Adriamycin (0.5ng/ml, 24hours). Lane 2 : extracts from HeLa cells treated
with Adriamycin (0.5ng/ml, 24hours), and treated with the immunising phosphopeptide.