Anti-Hormone sensitive lipase (phospho S855)抗体(ab55228)

概述

  • 产品名称Anti-Hormone sensitive lipase (phospho S855)抗体
    参阅全部 Hormone sensitive lipase 一抗
  • 描述
    兔多克隆抗体to Hormone sensitive lipase (phospho S855)
  • 特异性ab55228 detects endogenous levels of HSL/LIPE only when phosphorylated at serine 554.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human HSL/LIPE around the phosphorylation site of serine 554 (S-V-SP-E-A).

  • 阳性对照
    • extracts from HeLa cells treated with Adriamycin (0.5ng/ml, 24hours),

性能

应用

Our Abpromise guarantee covers the use of ab55228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 117 kDa).

靶标

Anti-Hormone sensitive lipase (phospho S855) antibody 图像

  • All lanes : Anti-Hormone sensitive lipase (phospho S855) antibody (ab55228) at 1/500 dilution

    Lane 1 : extracts from HeLa cells treated with Adriamycin (0.5ng/ml, 24hours).
    Lane 2 : extracts from HeLa cells treated with Adriamycin (0.5ng/ml, 24hours), and treated with the immunising phosphopeptide.

    Lysates/proteins at 5 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG (H+L)

    Predicted band size : 117 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

Anti-Hormone sensitive lipase (phospho S855) antibody (ab55228)参考文献

ab55228 has not yet been referenced specifically in any publications.

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