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RabMAb

Anti-Hormone sensitive lipase (phospho S853)抗体[EPR2329(2)] (ab109400)

概述

  • 产品名称
    Anti-Hormone sensitive lipase (phospho S853)抗体[EPR2329(2)]
    参阅全部 Hormone sensitive lipase 一抗
  • 描述
    兔单克隆抗体[EPR2329(2)] to Hormone sensitive lipase (phospho S853)
  • 特异性
    The antibody only detects HSL phosphorylated on serine 853.
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Phospho-specific peptide corresponding to residues surrounding serine 853 of Human HSL/LIPE (UniProt Q05469).

  • 阳性对照
    • Human skeletal muscle lysates and Mouse muscle lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

应用

Our Abpromise guarantee covers the use of ab109400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000 - 1/200000. Detects a band of approximately 83 kDa (predicted molecular weight: 117 kDa).
  • 应用说明
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    图片

    • All lanes : Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution

      Lane 1 : Human skeletal muscle lysates
      Lane 2 : Human skeletal muscle lysates treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 117 kDa
      Observed band size : 83 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution

      Lane 1 : Mouse muscle lysate
      Lane 2 : Mouse muscle lysate treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 117 kDa
      Observed band size : 83 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    ab109400 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Nov 05 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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