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Our Abpromise guarantee covers the use of ab45422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2.5 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa).|
ab45422 staining Hormone sensitive lipase in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG H&L (1/200) polyclonal was used as the secondary antibody. Nuclei were stained with DAPI.MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"