Anti-Hormone sensitive lipase抗体(ab45422)

概述

  • 产品名称Anti-Hormone sensitive lipase抗体
    参阅全部 Hormone sensitive lipase 一抗
  • 描述
    兔多克隆抗体to Hormone sensitive lipase
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic 14 amino acid peptide from near the carboxy terminus of human HSL/LIPE

  • 阳性对照
    • Human lymph node tissue lysate.

应用

Our Abpromise guarantee covers the use of ab45422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa).

靶标

Anti-Hormone sensitive lipase antibody 图像

  • ab45422 staining Hormone sensitive lipase in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG H&L (1/200) polyclonal was used as the secondary antibody. Nuclei were stained with DAPI.MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.

    See Abreview

  • Lane 1 : Anti-Hormone sensitive lipase antibody (ab45422) at 0.5 µg/ml
    Lane 2 : Anti-Hormone sensitive lipase antibody (ab45422) at 1 µg/ml

    Lane 1 : Human lymph node tissue lysate
    Lane 2 : Human lymph node tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 117 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa,86 kDa,88 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence of Lipe in Human Lymph Node cells using ab45422 at 20 ug/ml.

Anti-Hormone sensitive lipase antibody (ab45422)参考文献

This product has been referenced in:
  • Jiao J  et al. Chronic leucine supplementation improves lipid metabolism in C57BL/6J mice fed with a high-fat/cholesterol diet. Food Nutr Res 60:31304 (2016). WB ; Mouse . Read more (PubMed: 27616737) »
  • Ikoma-Seki K  et al. Role of LRP1 and ERK and cAMP Signaling Pathways in Lactoferrin-Induced Lipolysis in Mature Rat Adipocytes. PLoS One 10:e0141378 (2015). WB ; Rat . Read more (PubMed: 26506094) »

See all 8 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton-X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Nov 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"