• 产品名称Anti-Homer1抗体
    参阅全部 Homer1 一抗
  • 描述
    小鼠多克隆抗体to Homer1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length protein corresponding to Human Homer1.

  • 阳性对照
    • Rat brain tissue lysate, Homer transfected 293T cell lysate.



Our Abpromise guarantee covers the use of ab88827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).


  • 功能Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.
  • 序列相似性Belongs to the Homer family.
    Contains 1 WH1 domain.
  • 结构域The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
  • 细胞定位Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HOME1_HUMAN antibody
    • Homer antibody
    • HOMER 1A antibody
    • HOMER 1B antibody
    • HOMER 1C antibody
    • Homer homolog 1 (Drosophila) antibody
    • homer homolog 1 antibody
    • Homer protein homolog 1 antibody
    • homer scaffolding protein 1 antibody
    • Homer, Drosophila, homolog of, 1 antibody
    • Homer, neuronal immediate early gene, 1 antibody
    • Homer-1 antibody
    • Homer1 antibody
    • HOMER1A antibody
    • HOMER1B antibody
    • HOMER1C antibody
    • SYN47 antibody
    • Ves 1 antibody
    see all

Anti-Homer1 antibody 图像

  • Anti-Homer1 antibody (ab88827) at 1/500 dilution + rat brain tissue lysate at 50 µg

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • All lanes : Anti-Homer1 antibody (ab88827) at 1/500 dilution

    Lane 1 : Homer transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa

Anti-Homer1 antibody (ab88827)参考文献

ab88827 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Jurkat cells)
Specification Jurkat cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C

Ms. Poonam Singh

Verified customer

提交于 Jan 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293 cells)
Loading amount 20 µg
Specification 293 cells
Treatment siHomer1 siRNA
Gel Running Conditions Reduced Denaturing (12 % SDS PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jan 25 2013