Anti-Homer1抗体(ab88827)

概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to Homer1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length protein corresponding to Human Homer1.

  • 阳性对照
    • Rat brain tissue lysate, Homer transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab88827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

靶标

  • 功能
    Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.
  • 序列相似性
    Belongs to the Homer family.
    Contains 1 WH1 domain.
  • 结构域
    The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
  • 细胞定位
    Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HOME1_HUMAN antibody
    • Homer antibody
    • HOMER 1A antibody
    • HOMER 1B antibody
    • HOMER 1C antibody
    • Homer homolog 1 (Drosophila) antibody
    • homer homolog 1 antibody
    • Homer protein homolog 1 antibody
    • homer scaffolding protein 1 antibody
    • Homer, Drosophila, homolog of, 1 antibody
    • Homer, neuronal immediate early gene, 1 antibody
    • Homer-1 antibody
    • Homer1 antibody
    • HOMER1A antibody
    • HOMER1B antibody
    • HOMER1C antibody
    • SYN47 antibody
    • Ves 1 antibody
    see all

Anti-Homer1 antibody 图像

  • Anti-Homer1 antibody (ab88827) at 1/500 dilution + rat brain tissue lysate at 50 µg

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • All lanes : Anti-Homer1 antibody (ab88827) at 1/500 dilution

    Lane 1 : Homer transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 39 kDa
    Observed band size : 39 kDa

实验方案

Anti-Homer1 antibody (ab88827)参考文献

ab88827 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Jurkat cells)
Specification
Jurkat cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Ms. Poonam Singh

Verified customer

提交于 Jan 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293 cells)
Loading amount
20 µg
Specification
293 cells
Treatment
siHomer1 siRNA
Gel Running Conditions
Reduced Denaturing (12 % SDS PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 25 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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