Anti-Homer (1b+1c)抗体(ab97593)

概述

  • 产品名称Anti-Homer (1b+1c)抗体
  • 描述
    兔多克隆抗体to Homer (1b+1c)
  • 经测试应用适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 72 and 309 of Human Homer (1b+1c) (NP_004263)

  • 阳性对照
    • 293T, A431, H1299, HepG2 or Molt-4 cell lysate OVCA tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab97593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/250.
IHC-Fr 1/200.

靶标

  • 相关性Homer is a neuronal immediate early gene that is enriched at excitatory synapses and selectively binds metabotropic glutamate receptors.
  • 细胞定位Cytoplasmic; Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Postsynaptic density of neuronal cells. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.
  • 数据库链接
  • 别名
    • Homer 1 antibody
    • HOMER antibody
    • Homer homolog 1 antibody
    • Homer protein homolog 1 antibody
    • Homer1 antibody
    • HOMER1B antibody
    • HOMER1C antibody
    • Neuronal immediate early gene antibody
    • SYN47 antibody
    • Ves 1 antibody
    see all

Anti-Homer (1b+1c) antibody 图像

  • All lanes : Anti-Homer (1b+1c) antibody (ab97593) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 40 kDa
    Gel conc 10% (SDS PAGE)
  • ab97593 staining Homer (1b+1c) in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, blocked with 1% BSA for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 1% BSA + 0.1% Tx-PBS) for 16 hours at 25°C. An Alexa Flour® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

  • ab97593, at a 1/100 dilution, staining human Homer (1b+1c) in OVCA, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

Anti-Homer (1b+1c) antibody (ab97593)参考文献

ab97593 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Rat Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Oct 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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Mr. Yahyah Aman

Verified customer

提交于 Dec 02 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse spinal cord)
Permeabilization Yes - 0.1% PBS Triton X-100
Specification Mouse spinal cord
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 18 2015

Application Flow Cytometry
Sample Human Cell (Differentiated hNSCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Undifferentiated Stem Cells (white)
Specification Differentiated hNSCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 11 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (Differentiated hNSCs)
Specification Differentiated hNSCs
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 17 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"