Homer is a neuronal immediate early gene that is enriched at excitatory synapses and selectively binds metabotropic glutamate receptors.
Cytoplasmic; Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Postsynaptic density of neuronal cells. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.
Western blot - Anti-Homer (1b+1c) antibody (ab97593)
All lanes : Anti-Homer (1b+1c) antibody (ab97593) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
Gel conc 10% (SDS PAGE)
Immunohistochemistry (Frozen sections) - Anti-Homer (1b+1c) antibody (ab97593)Image courtesy of an anonymous Abreview.
ab97593 staining Homer (1b+1c) in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, blocked with 1% BSA for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 1% BSA + 0.1% Tx-PBS) for 16 hours at 25°C. An Alexa Flour® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.