Anti-HNRPH3抗体(ab66663)

概述

  • 产品名称Anti-HNRPH3抗体
    参阅全部 HNRPH3 一抗
  • 描述
    兔多克隆抗体to HNRPH3
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: GLEIVPNGIT LTMDYQGRST GEAFVQFASK EIAENALGKH KERIGHRYIE , corresponding to amino acids 38-87 of Human HNRPH3

  • 阳性对照
    • Raji cell lysate. Human kidney tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

  • 相关性HNRPH3 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • 2H9 antibody
    • FLJ34092 antibody
    • Heterogeneous nuclear ribonucleoprotein H3 antibody
    • hnRNP 2H9 antibody
    • hnRNP H3 antibody
    • HNRNPH 3 antibody
    • HNRNPH3 antibody
    • HNRPH 3 antibody
    see all

Anti-HNRPH3 antibody 图像

  • All lanes : Anti-HNRPH3 antibody (ab66663) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Raji cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 17 kDa,35 kDa (possible isoform). We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human kidney tissue labelling HNRPH3 with ab66663 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnifciation: 400X.

Anti-HNRPH3 antibody (ab66663)参考文献

ab66663 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control hnRNP C (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control Protein A dynabeads without antibody bound
Immuno-precipitation step Other - Protein A Dynabeads
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提交于 Jun 02 2011

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