概述

  • 产品名称Anti-HNRPDL抗体
    参阅全部 HNRPDL 一抗
  • 描述
    兔多克隆抗体to HNRPDL
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide designed within residues: YSGYGGYDYT GYNYGNYGYG QGYADYSGQQ STYGKASRGG GNHQNNYQPY, corresponding to C terminal amino acids 372-421 of Human HNRPDL (NP_005454)

  • 阳性对照
    • MCF7 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Acts as a transcriptional regulator. Promotes transcription repression. Promotes transcription activation in differentiated myotubes (By similarity). Binds to double- and single-stranded DNA sequences. Binds to the transcription suppressor CATR sequence of the COX5B promoter (By similarity). Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Binds both to nuclear and cytoplasmic poly(A) mRNAs. Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers. Binds to the 5'-ACUAGC-3' RNA consensus sequence.
  • 组织特异性Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Expressed in myeloid leukemia, gastric adenocarcinoma, cervical carcinoma, hepatoma, fibrosarcoma, colon adenocarcinoma, epidermoid carcinoma, osteosarcoma and urinary bladder carcinoma cells.
  • 序列相似性Contains 2 RRM (RNA recognition motif) domains.
  • 翻译后修饰Dimethylation of Arg-408 is probably of the asymmetric type.
  • 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm in a TNPO1-dependent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A+U-rich element RNA binding factor antibody
    • AA407431 antibody
    • AA959857 antibody
    • AU rich element RNA binding factor antibody
    • AU-rich element RNA-binding factor antibody
    • D5Ertd650e antibody
    • D5Wsu145e antibody
    • Heterogeneous nuclear ribonucleoprotein D like antibody
    • Heterogeneous nuclear ribonucleoprotein D like protein antibody
    • Heterogeneous nuclear ribonucleoprotein D-like antibody
    • hnHNRP DL antibody
    • HNRDL_HUMAN antibody
    • HNRNP antibody
    • hnRNP D-like antibody
    • hnRNP DL antibody
    • HNRNPDL antibody
    • hnRPD like protein antibody
    • JKT41 binding protein antibody
    • JKT41-binding protein antibody
    • JKTBP antibody
    • JKTBP2 antibody
    • laAUF1 antibody
    • MGC125262 antibody
    • Protein laAUF1 antibody
    see all

Anti-HNRPDL antibody 图像

  • Anti-HNRPDL antibody (ab83215) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab83215 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83215, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HNRPDL antibody (ab83215)参考文献

This product has been referenced in:
  • Zhou H  et al. Growth arrest specific 2 is up-regulated in chronic myeloid leukemia cells and required for their growth. PLoS One 9:e86195 (2014). WB ; Human . Read more (PubMed: 24465953) »
  • Li X  et al. Suppression of HPV-16 late L1 5'-splice site SD3632 by binding of hnRNP D proteins and hnRNP A2/B1 to upstream AUAGUA RNA motifs. Nucleic Acids Res N/A:N/A (2013). WB ; Human . Read more (PubMed: 24013563) »

See all 3 Publications for this product

Product Wall

Thank you for your inquiry.

I can confirm that ab82968 and ab92355 both can be used with citrate buffer pH6 as antigen retrieval. That is according to a n Abreview and lab testing respectively.

ab83215 is not tested and guaranteed f...

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