Anti-HNRPA3/HNRNPA3抗体(ab78300)
Key features and details
- Rabbit polyclonal to HNRPA3/HNRNPA3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-HNRPA3/HNRNPA3抗体 -
描述
兔多克隆抗体to HNRPA3/HNRNPA3 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; Raji; SHSY-5Y; Caco 2.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78300于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
IHC-P |
Use a concentration of 1 µg/ml.
|
|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa).
|
说明 |
---|
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa). |
靶标
-
相关性
HNRPA3 plays a role in cytoplasmic trafficking of RNA. It binds to the cis-acting response element(A2RE) and may be involved in pre-mRNA splicing. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 220988 Human
- Entrez Gene: 229279 Mouse
- Entrez Gene: 362152 Rat
- Omim: 605372 Human
- SwissProt: P51991 Human
- SwissProt: Q8BG05 Mouse
- SwissProt: Q6URK4 Rat
-
别名
- FBRNP antibody
- Heterogeneous nuclear ribonucleoprotein A3 antibody
- HNRPA3 antibody
图片
-
All lanes : Anti-HNRPA3/HNRNPA3 antibody (ab78300) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteHNRPA3/HNRNPA3 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
-
IHC image of ab78300 staining in Human Cervical Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78300, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab78300 被引用在 1 文献中.
- Kazuma Y et al. ILF2 enhances the DNA cytosine deaminase activity of tumor mutator APOBEC3B in multiple myeloma cells. Sci Rep 12:2278 (2022). PubMed: 35145187