概述

  • 产品名称Anti-HNRPA3抗体
    参阅全部 HNRPA3 一抗
  • 描述
    兔多克隆抗体to HNRPA3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Dog
  • 免疫原

    A region within synthetic peptide: MEVKPPPGRP QPDSGRRRRR RGEEGHDPKE PEQLRKLFIG GLSFETTDDS, corresponding to N terminal amino acids 1-50 of Human HNRPA3

  • 阳性对照
    • IHC-P: Human liver and human lung tissues WB: Jurkat cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab50949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

Anti-HNRPA3 antibody 图像

  • All lanes : Anti-HNRPA3 antibody (ab50949) at 0.625 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 40 kDa
  • Tissue: Paraffin embedded human liver
    Cells with positive label: Hepatocytes (indicated with arrows)
    Antibody concentration: 4µg/ml
    Magnification: 400X
    Tissue: Paraffin embedded human liver
    Cells with positive label: Hepatocytes (indicated with arrows)
    Antibody concentration: 4µg/ml
    Magnification: 400X
  • Anti-HNRPA3 antibody (ab50949) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-rabbit IgG at 1/50,000

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Buffer: 5% skim milk / PBS
    Gel concentration: 12%
  • Tissue: Paraffin-embedded human lung
    Cells with positive label: Alveolar cells (indicated with arrows)
    Antibody concentration: 4 µg/ml
    Magnification: 400X
    Tissue: Paraffin-embedded human lung
    Cells with positive label: Alveolar cells (indicated with arrows)
    Antibody concentration: 4 µg/ml
    Magnification: 400X

Anti-HNRPA3 antibody (ab50949)参考文献

This product has been referenced in:
  • Roda D  et al. EGF-Induced Acetylation of Heterogeneous Nuclear Ribonucleoproteins Is Dependent on KRAS Mutational Status in Colorectal Cancer Cells. PLoS One 10:e0130543 (2015). Human . Read more (PubMed: 26110767) »
  • Papadopoulou C  et al. Extensive association of HuR with hnRNP proteins within immunoselected hnRNP and mRNP complexes. Biochim Biophys Acta 1804:692-703 (2010). Read more (PubMed: 19931428) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount 50 µg
Specification Mouse embryonic fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"