概述

  • 产品名称Anti-HNRNPA0抗体
    参阅全部 HNRNPA0 一抗
  • 描述
    兔多克隆抗体to HNRNPA0
  • 经测试应用适用于: WB, IHC-P, IHC-FoFr, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: YFQNHDAADK AAVVKFHPIQ GHRVEVKKAV PKEDIYSGGG GGGSRSSRGG, corresponding to internal sequence amino acids 145-194 of Human HNRNPA0

  • 阳性对照
    • Human heart and liver tissue sections and Jurkat cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab66661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.625 µg/ml. Detects a band of approximately 34, 36 kDa (predicted molecular weight: 31 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21970342
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration. ELISA titre using peptide based assay 1:312500.

靶标

Anti-HNRNPA0 antibody 图像

  • Anti-HNRNPA0 antibody (ab66661) at 0.625 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab66661 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66661, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HNRNPA0 with ab66661 at 4-8µg/ml. Arrows indicate positively labelled hepatocyte cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling HNRNPA0 with ab6661 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.

Anti-HNRNPA0 antibody (ab66661)参考文献

This product has been referenced in:
  • Naji S  et al. Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production. Mol Cell Proteomics 11:M111.015313 (2012). WB . Read more (PubMed: 22174317) »
  • Ranjbarvaziri S  et al. Quantum dot labeling using positive charged peptides in human hematopoetic and mesenchymal stem cells. Biomaterials 32:5195-205 (2011). IHC-P ; Human . Read more (PubMed: 21549422) »

See all 3 Publications for this product

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