Anti-hnRNP U抗体[3G6] (ab10297)

概述

  • 产品名称Anti-hnRNP U抗体[3G6]
    参阅全部 hnRNP U 一抗
  • 描述
    小鼠单克隆抗体[3G6] to hnRNP U
  • 经测试应用适用于: ELISA, WB, IP, ICC/IF, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
    不与反应: Chicken, Lizard
  • 免疫原

    Recombinant full length protein (Human).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Protein A purified from tissue culture supernatant.
  • 克隆单克隆
  • 克隆编号3G6
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 120 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/100.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.
  • 序列相似性Contains 1 B30.2/SPRY domain.
    Contains 1 SAP domain.
  • 翻译后修饰Extensively phosphorylated.
    Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位Nucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heterogeneous nuclear ribonucleoprotein U antibody
    • hnRNP U antibody
    • hnRNP U protein antibody
    • HNRNPU antibody
    • hnRNPU protein antibody
    • HNRPU antibody
    • HNRPU_HUMAN antibody
    • p120 antibody
    • p120 nuclear protein antibody
    • pp120 antibody
    • SAF A antibody
    • SAF-A antibody
    • SAFA antibody
    • Scaffold attachment factor A antibody
    • U21.1 antibody
    see all

Anti-hnRNP U antibody [3G6] 图像

  • All lanes : Anti-hnRNP U antibody [3G6] (ab10297) at 1/1000 dilution

    Lane 1 : Cytoplasmic fraction of HeLa lysate
    Lane 2 : Nuclear fraction of HeLa lysate


    Predicted band size : 120 kDa
    Observed band size : 120 kDa
  • ab10297 at 1/100 staining U20S cells. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A FITC conjugated anti-mouse antibody was used as the secondary. The cells were counterstained with DAPI (left hand panel).

    See Abreview

  • Anti-hnRNP U antibody [3G6] (ab10297) at 1/5000 dilution + Human 293T - whole cell lysate at 100 µg

    Secondary
    HRP conjugated sheep anti-mouse IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 120 kDa
    Additional bands at : 60 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov

    See Abreview

  • ab10297 (4µg/ml)) staining hnRNPU in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining of Purkinje cells neurons.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Overlay histogram showing HeLa cells stained with ab10297 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10297, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-hnRNP U antibody [3G6] (ab10297)参考文献

This product has been referenced in:
  • Meininger I  et al. Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells. Nat Commun 7:11292 (2016). Mouse . Read more (PubMed: 27068814) »
  • Majerciak V  et al. Stability of structured Kaposi's sarcoma-associated herpesvirus ORF57 protein is regulated by protein phosphorylation and homodimerization. J Virol 89:3256-74 (2015). Read more (PubMed: 25568207) »

See all 10 Publications for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Sample Human Cell lysate - whole cell (Human Embryonic Kidney)
Specification Human Embryonic Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 01 2014

Thank you for confirming those details.

I'd be happy to arrange for a credit note to be issued. In order to do this, could you please provide the order number on which ab10297 was purchased?

Many thanks in advance for your cooperation.

Thank you very much for that information.

I understand the frustration the customer must be feeling and will try to help them as quickly as I can. May I just ask which xxxxxx antibody was used, and which secondary antibody?

Has the ...

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Thank you for contacting us and reporting the problems encountered with the anti-hnRNP U antibody [3G6] (ab10297).

I am sorry to hear youhave beenexperiencing difficulties with one of our products. We take product complaints very seriously, ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela cytosolic and nuclear extracts)
Loading amount 50 µg
Specification Hela cytosolic and nuclear extracts
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Nov 12 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 100 µg
Specification 293T
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Stoil Dmitrov

Verified customer

提交于 Apr 09 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS osteosarcoma line)
Specification U2OS osteosarcoma line
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1%
Username

Dr. Stoil Dmitrov

Verified customer

提交于 Apr 06 2007

Thank you for your enquiry. I am sorry to hear you are having a problem with ab10297. I am assuming the proportion of antibody to lysate is appropriate. For the 5 ug of antibody you are using, I suggest incubating with no more than 500 ug lysate, a...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Following a literature search I could not find evidence for the enrichment of hnRNP U on the female inactive X chromosome. According to Ve...

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I received today the following information from the originator of ab10297: "We have run our own western on HeLa extract which confirms that the major band is at ~120-125kDa (see blot attached). This corresponds with the information published in the re...

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