概述

  • 产品名称Anti-hnRNP R抗体
    参阅全部 hnRNP R 一抗
  • 描述
    兔多克隆抗体to hnRNP R
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human hnRNP R.

    (Peptide available as ab31802.)

  • 阳性对照
    • HeLa (Human epithelial carcinoma cell line) Nuclear Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Testis (Mouse) Tissue Lysate - normal tissue Ovary (Mouse) Tissue Lysate - normal tissue PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab30930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/1500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500. Detects a band of approximately 68, 73, 76 kDa (predicted molecular weight: 71 kDa).

靶标

Anti-hnRNP R antibody 图像

  • Anti-hnRNP R antibody (ab30930) at 1/500 dilution + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 68,73,76 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-hnRNP R antibody (ab30930) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 73,77,80 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab30930 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab30930, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Image courtesy of Human Protein Atlas. ab30930 staining hnRNP R in human stomach, showing a strong nuclear staining pattern in all cells. Paraffin embedded human stomach tissue was incubated with ab30930 (1/1500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab30930 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

Anti-hnRNP R antibody (ab30930)参考文献

This product has been referenced in:
  • Dombert B  et al. Presynaptic localization of Smn and hnRNP R in axon terminals of embryonic and postnatal mouse motoneurons. PLoS One 9:e110846 (2014). Read more (PubMed: 25338097) »
  • Glinka M  et al. The heterogeneous nuclear ribonucleoprotein-R is necessary for axonal {beta}-actin mRNA translocation in spinal motor neurons. Hum Mol Genet : (2010). Zebrafish . Read more (PubMed: 20167579) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% resolving gel)
Sample Human Cell lysate - whole cell (Fibroblasts)
Specification Fibroblasts
Username

Mr. Yongchul Lim

Verified customer

提交于 Jun 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"