概述

  • 产品名称Anti-hnRNP Q抗体[I8E4]
    参阅全部 hnRNP Q 一抗
  • 描述
    小鼠单克隆抗体[I8E4] to hnRNP Q
  • 经测试应用适用于: ICC/IF, Flow Cyt, ELISA, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human, Xenopus laevis
  • 免疫原

    Recombinant untagged hnRNP-Q protein (human).

性能

应用

Our Abpromise guarantee covers the use of ab10687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 55,60,70 kDa (predicted molecular weight: 70 kDa).
IP Use at an assay dependent concentration.

靶标

  • 功能Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.
  • 组织特异性Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.
  • 序列相似性Contains 3 RRM (RNA recognition motif) domains.
  • 结构域The domain containing eight Arg-Gly-Gly repeats may be involved in RNA-binding and protein-protein interactions.
  • 翻译后修饰Phosphorylated on tyrosine. The membrane-bound form found in microsomes is phosphorylated in vitro by insulin receptor tyrosine kinase (INSR). Phosphorylation is inhibited upon binding to RNA, whereas the cytoplasmic form is poorly phosphorylated (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > nucleoplasm. Expressed predominantly in the nucleoplasm and Cytoplasm. Microsome. Endoplasmic reticulum. Nucleus. The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cytoplasmic RNA-interacting protein antibody
    • dJ3J17.2 antibody
    • Glycine and tyrosine rich RNA binding protein antibody
    • Glycine- and tyrosine-rich RNA-binding protein antibody
    • GRY RBP antibody
    • GRY-RBP antibody
    • GRYRBP antibody
    • Heterogeneous nuclear ribonucleoprotein Q antibody
    • hnRNP Q antibody
    • HNRPQ antibody
    • HNRPQ_HUMAN antibody
    • HNRPQ1 antibody
    • NS1 associated protein 1 antibody
    • NS1-associated protein 1 antibody
    • NSAP1 antibody
    • pp68 antibody
    • RP1 3J17.2 antibody
    • Synaptotagmin binding cytoplasmic RNA interacting protein antibody
    • Synaptotagmin-binding antibody
    • Syncrip antibody
    see all

Anti-hnRNP Q antibody [I8E4] 图像

  • ab10687 staining hnRNP Q in the HT1080 cell line from Human fibrosarcoma biopsy by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were transfected with scrRNA and then the cells were fixed with ice-cold methanol.  Samples were incubated with primary antibody. An Alexa Fluor®488-conjugated Donkey anti-mouse  was used as the secondary antibody. DNA staining was performed using DAPI.

  • Overlay histogram showing HeLa cells stained with ab10687 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10687, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-hnRNP Q antibody [I8E4] (ab10687)参考文献

This product has been referenced in:
  • Katoh H  et al. Japanese Encephalitis Virus Core Protein Inhibits Stress Granule Formation through an Interaction with Caprin-1 and Facilitates Viral Propagation. J Virol 87:489-502 (2013). WB . Read more (PubMed: 23097442) »
  • Peddigari S  et al. hnRNPL and nucleolin bind LINE-1 RNA and function as host factors to modulate retrotransposition. Nucleic Acids Res 41:575-85 (2013). WB ; Human . Read more (PubMed: 23161687) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human fibroblast)
Loading amount 20 µg
Specification Human fibroblast
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Nupage)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 20 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (10%)
Blocking step 1% milk, 1% BSA in TBST as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Ms. Katharine Grabek

Verified customer

提交于 Jul 14 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human lymphoblastoid cell line GM10725)
Specification Human lymphoblastoid cell line GM10725
Fixative Formaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Apr 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"