Anti-hnRNP Q抗体(ab58854)

概述

  • 产品名称
    Anti-hnRNP Q抗体
    参阅全部 hnRNP Q 一抗
  • 描述
    兔多克隆抗体to hnRNP Q
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: EGENIEIVFA KPPDQKRKER KAQRQAAKNQ MYDDYYYYGP PHMPPPTRGR, corresponding to internal sequence amino acids 397-446 of Human hnRNP Q

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab58854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 70 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.
  • 组织特异性
    Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.
  • 序列相似性
    Contains 3 RRM (RNA recognition motif) domains.
  • 结构域
    The domain containing eight Arg-Gly-Gly repeats may be involved in RNA-binding and protein-protein interactions.
  • 翻译后修饰
    Phosphorylated on tyrosine. The membrane-bound form found in microsomes is phosphorylated in vitro by insulin receptor tyrosine kinase (INSR). Phosphorylation is inhibited upon binding to RNA, whereas the cytoplasmic form is poorly phosphorylated (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus > nucleoplasm. Expressed predominantly in the nucleoplasm and Cytoplasm. Microsome. Endoplasmic reticulum. Nucleus. The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cytoplasmic RNA-interacting protein antibody
    • dJ3J17.2 antibody
    • Glycine and tyrosine rich RNA binding protein antibody
    • Glycine- and tyrosine-rich RNA-binding protein antibody
    • GRY RBP antibody
    • GRY-RBP antibody
    • GRYRBP antibody
    • Heterogeneous nuclear ribonucleoprotein Q antibody
    • hnRNP Q antibody
    • HNRPQ antibody
    • HNRPQ_HUMAN antibody
    • HNRPQ1 antibody
    • NS1 associated protein 1 antibody
    • NS1-associated protein 1 antibody
    • NSAP1 antibody
    • pp68 antibody
    • RP1 3J17.2 antibody
    • Synaptotagmin binding cytoplasmic RNA interacting protein antibody
    • Synaptotagmin-binding antibody
    • Syncrip antibody
    see all

Anti-hnRNP Q antibody 图像

  • Anti-hnRNP Q antibody (ab58854) at 1.25 µg/ml + HepG2 cell lysate at 10µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-hnRNP Q antibody (ab58854) at 1/800 dilution

    Lane 1 : NSC34 whole cell lysate with control siRNA
    Lane 2 : NSC34 whole cell lysate with hnRNP Q1 siRNA

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye® 800CW-conjugated Donkey anti-Mouse IgG at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 70 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

实验方案

Anti-hnRNP Q antibody (ab58854)参考文献

ab58854 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NSC34 cells)
Loading amount
10 µg
Specification
NSC34 cells
Gel Running Conditions
Reduced Denaturing (4-12% Nugage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Nov 12 2010

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