Anti-hnRNP M3-M4抗体(ab55486)

概述

  • 产品名称Anti-hnRNP M3-M4抗体
    参阅全部 hnRNP M3-M4 一抗
  • 描述
    小鼠单克隆抗体to hnRNP M3-M4
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: KMEEESGAPG VPSGNGAPGP KGEGERPAQN EKRKEKNIKR GGNRFEPYAN PTKRYRAFIT NIPFDVKWQS LKDLVKEKVG EVTYVELLMD AEGKSR, corresponding to amino acids 17-113 of Human hnRNP M3-M4

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab55486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 77 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
  • 序列相似性Contains 3 RRM (RNA recognition motif) domains.
  • 翻译后修饰Sumoylated.
  • 细胞定位Nucleus, nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CEA receptor antibody
    • CEAR antibody
    • Heterogeneous nuclear ribonucleoprotein M antibody
    • Heterogenous nuclear ribonucleoprotein M4 antibody
    • hnRNA binding protein M4 antibody
    • hnRNP M antibody
    • Hnrnpm antibody
    • HNRNPM4 antibody
    • HNRPM antibody
    • HNRPM_HUMAN antibody
    • HNRPM4 antibody
    • HTGR1 antibody
    • M3 antibody
    • M4 antibody
    • M4 protein antibody
    • N-acetylglucosamine receptor 1 antibody
    • NAGR1 antibody
    see all

Anti-hnRNP M3-M4 antibody 图像

  • hnRNP M3-M4 antibody (ab55486) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human thyroid nodular goiter.


  • Predicted band size : 77 kDa
    hnRNP M3-M4 antibody (ab55486) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
  • ab55486 at 10 ug/ml staining HNRPM in human HepG2 cells by Immunocytochemistry/ Immunofluorescence.

Anti-hnRNP M3-M4 antibody (ab55486)参考文献

ab55486 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (293 cell total extract)
Total protein in input 1000 µg
Specification 293 cell total extract
Immuno-precipitation step Protein A
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提交于 Oct 17 2011

Application Western blot
Sample Human Cell lysate - whole cell (HCT116 cells)
Loading amount 50 µg
Specification HCT116 cells
Gel Running Conditions Reduced Non-Denaturing (Native) (6-15% gradient gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Oct 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"